Commit d0f3c53d authored by TomKellyGenetics's avatar TomKellyGenetics
Browse files

add support for inDrops version 3 configuration

parent 4e8b162f
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+14 −3
Original line number Diff line number Diff line
@@ -77,6 +77,7 @@ Mandatory arguments to long options are mandatory for short options too.
                                  iCell8 version 3 (11bp barcode, 14bp UMI): icell8 or custom
                                  inDrops version 1 (19bp barcode, 8bp UMI): indrops-v1
                                  inDrops version 2 (19bp barcode, 8bp UMI): indrops-v2 
                                  inDrops version 3 (8bp barcodes, 6bp UMI): indrops-v3
                                  Quartz-Seq2 (14bp barcode, 8bp UMI): quartzseq2-384
                                  Quartz-Seq2 (15bp barcode, 8bp UMI): quartzseq2-1536
                                  Sci-Seq (8bp UMI, 10bp barcode): sciseq
@@ -467,7 +468,14 @@ fi
if [[ "$technology" == "indrop-v2" ]] || [[ "$technology" == "indrops-v2" ]] || [[ "$technology" == "indropv2" ]] || [[ "$technology" == "indropsv2" ]]; then
    echo "Running with inDrop parameters (version 2 with reads inverted)"
    technology="indrop-v2"    
fi
if [[ "$technology" == "indrop-v3" ]] || [[ "$technology" == "indrops-v3" ]] || [[ "$technology" == "indropv3" ]] || [[ "$technology" == "indropsv3" ]]; then
    echo "Running with inDrop parameters (version 3 with reads inverted)"
    technology="indrop-v3"
fi
if [[ "$technology" == "indrop-v2" ]] || [[ "$technology" == "indrop-v3" ]]; then
    #invert read1 and read2
    echo "Using barcodes on Read 2"
    tmp=$read1
    read1=$read2
    read2=$tmp
@@ -921,7 +929,10 @@ elif [[ "$technology" == "icell8" ]]; then
    umilength=14
elif [[ "$technology" == "indrop-v1" ]] || [[ "$technology" == "indrop-v2" ]]; then
    barcodelength=19 
    umilength=8
    umilength=6
elif [[ "$technology" == "indrop-v3" ]]; then
    barcodelength=8
    umilength=6
elif [[ "$technology" == "quartz-seq2-384" ]]; then
    barcodelength=14
    umilength=8