Commit 84dd5560 authored by TomKellyGenetics's avatar TomKellyGenetics
Browse files

add example for ICELL8 with custom barcodes

parent d51fd885
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+11 −1
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@@ -923,7 +923,17 @@ bash universc/launch_universc.sh --id "test-dropseq" --technology "nadia" \

#### Running launch_universc.sh on ICELL8 data

- add example running on custom barcode whitelist
```
# call on icell8 files with custom whitelist and non-standard file names
bash launch_universc.sh --setup -t "icell8"  --barcodefile "test/shared/icell8-test/BarcodeList.txt"
bash launch_universc.sh --id "test-icell8-custom" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/iCELL8_01_S1_L001_R1_001.fastq" "test/shared/icell8-test/iCELL8_01_S1_L002_R1_001.fastq" \
 --read2 "test/shared/icell8-test/iCELL8_01_S1_L001_R2_001.fastq" "test/shared/icell8-test/iCELL8_01_S1_L002_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/BarcodeList.txt" \
 --jobmode "sge"
```


## Licensing

+70 −278

File changed.

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+13 −3
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@@ -6,7 +6,7 @@ affiliations:
   index: 1
 - name: "RIKEN Center for Sustainable Resource Sciences, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Kanagawa 230-0045, Japan"
   index: 2
date: "Thursday 09 July 2020"
date: "Sunday 12 July 2020"
output:
  prettydoc::html_pretty:
       theme: cayman
@@ -923,7 +923,17 @@ bash universc/launch_universc.sh --id "test-dropseq" --technology "nadia" \

#### Running launch_universc.sh on ICELL8 data

- add example running on custom barcode whitelist
```
# call on icell8 files with custom whitelist and non-standard file names
bash launch_universc.sh --setup -t "icell8"  --barcodefile "test/shared/icell8-test/BarcodeList.txt"
bash launch_universc.sh --id "test-icell8-custom" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/iCELL8_01_S1_L001_R1_001.fastq" "test/shared/icell8-test/iCELL8_01_S1_L002_R1_001.fastq" \
 --read2 "test/shared/icell8-test/iCELL8_01_S1_L001_R2_001.fastq" "test/shared/icell8-test/iCELL8_01_S1_L002_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/BarcodeList.txt" \
 --jobmode "sge"
```


## Licensing