Commit 42e1e07a authored by TomKellyGenetics's avatar TomKellyGenetics
Browse files

add tests for published ICELL8 data

parent 2beb4f0f
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+68 −1
Original line number Original line Diff line number Diff line
@@ -6,7 +6,7 @@
cd $(dirname ${BASH_SOURCE[0]})/..
cd $(dirname ${BASH_SOURCE[0]})/..


# used to export to PATH for testing on SGE server
# used to export to PATH for testing on SGE server
#export PATH=${HOME}/local/bin/cellranger-3.0.2:$PATH
export PATH=${HOME}/local/bin/cellranger-3.0.2:$PATH


cellrangerversion=`cellranger count --version | head -n 2 | tail -n 1 | cut -f2 -d'(' | cut -f1 -d')'`
cellrangerversion=`cellranger count --version | head -n 2 | tail -n 1 | cut -f2 -d'(' | cut -f1 -d')'`
cellrangerpath=`which cellranger`
cellrangerpath=`which cellranger`
@@ -37,3 +37,70 @@ bash launch_universc.sh --id "test-icell8-custom" --technology "iCell8" \
if [[ -f test/shared/icell8-test/*fastq ]]; then
if [[ -f test/shared/icell8-test/*fastq ]]; then
    gzip -f test/shared/icell8-test/*fastq
    gzip -f test/shared/icell8-test/*fastq
fi
fi

# call on icell8 files with custom whitelist and non-standard file names
bash launch_universc.sh --setup -t "icell8"  --barcodefile "test/shared/icell8-test/BarcodeList.txt"
bash launch_universc.sh --id "test-icell8-72618_A375-2-lanes" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_A375_L001_R1_001.fastq" "test/shared/icell8-test/72618_A375_L002_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_A375_L001_R2_001.fastq" "test/shared/icell8-test/72618_A375_L002_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"
bash launch_universc.sh --id "test-icell8-72618_A375-1-lane" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_A375_L001_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_A375_L001_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"

bash launch_universc.sh --id "test-icell8-72618_HCT116-2-lanes" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_HCT116_L001_R1_001.fastq" "test/shared/icell8-test/72618_HCT116_L002_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_HCT116_L001_R2_001.fastq" "test/shared/icell8-test/72618_HCT116_L002_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"
bash launch_universc.sh --id "test-icell8-72618_HCT116-2-lanes" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_HCT116_L001_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_HCT116_L001_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"

bash launch_universc.sh --id "test-icell8-72618_KU812-2-lanes" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_KU812_L001_R1_001.fastq" "test/shared/icell8-test/72618_KU812_L002_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_KU812_L001_R2_001.fastq" "test/shared/icell8-test/72618_KU812_L002_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"
bash launch_universc.sh --id "test-icell8-72618_KU812-1-lane" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_KU812_L001_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_KU812_L001_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"

bash launch_universc.sh --id "test-icell8-72618_NCI-H2452-2-lanes" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_NCI-H2452_L001_R1_001.fastq" "test/shared/icell8-test/72618_NCI-H2452_L002_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_NCI-H2452_L001_R2_001.fastq" "test/shared/icell8-test/72618_NCI-H2452_L002_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"
bash launch_universc.sh --id "test-icell8-72618_NCI-H2452-1-lane" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/72618_NCI-H2452_L001_R1_001.fastq" \
 --read2 "test/shared/icell8-test/72618_NCI-H2452_L001_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"

bash launch_universc.sh --id "test-icell8-test-2-lanes" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/test_L001_R1_001.fastq" "test/shared/icell8-test/test_L002_R1_001.fastq" \
 --read2 "test/shared/icell8-test/test_L001_R2_001.fastq" "test/shared/icell8-test/test_L002_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"
bash launch_universc.sh --id "test-icell8-test-1-lane" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/icell8-test/test_L001_R1_001.fastq" \
 --read2 "test/shared/icell8-test/test_L001_R2_001.fastq" \
 --barcodefile "test/shared/icell8-test/WellList.txt" \
 --jobmode "sge"