Commit 3c15bcd7 authored by TomKellyGenetics's avatar TomKellyGenetics
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test smart-seq3 with custom barcode whitelists

parent cdb980d9
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+4 −4
Original line number Diff line number Diff line
@@ -35,14 +35,14 @@ if [[ -d test-smartseq3 ]]; then
    rm -rf test-smartseq3
fi

whitelistdir=whitelists
join -j 9999 ${whitelistdir}/Aligent_SureSelectXT_Index1_i7_barcodes.txt  ${whitelistdir}/Aligent_SureSelectXT_Index2_i5_barcodes.txt | sed "s/ //g" > ${whitelistdir}/Aligent_SureSelectXT_dual_barcodes.txt

if [[ ! -f SmartSeq3_test_barcodes.txt ]]; then
    gunzip -k SmartSeq3_test_barcodes.txt.gz
fi

# call on smartseq3 with files
bash launch_universc.sh --id "test-smartseq3" --technology "smartseq" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/smartseq3-test/Smartseq3_diySpike_R1" \
 --read2 "test/shared/smartseq3-test/Smartseq3_diySpike_R2" \
 --barcodefile "${whitelistdir}/Aligent_SureSelectXT_dual_barcodes.txt" \
 --barcodefile "${whitelistdir}/SmartSeq3_test_barcodes.txt" \
 --jobmode "local" --localcores 1 
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