Commit 221b86a2 authored by TomKellyGenetics's avatar TomKellyGenetics
Browse files

correct test scripts to run automatically

parent 3502f127
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+16 −2
Original line number Diff line number Diff line
@@ -42,6 +42,9 @@ if [[ ! -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barco
    gzip -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt
fi
rm -rf ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt
if [[ -d tiny-test ]]; then
    rm -rf tiny-test
fi
# test cellranger call
cellranger testrun --id="tiny-test"
# unzip input data
@@ -58,7 +61,9 @@ fi
if [[ ! -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt.gz ]]; then
    gzip -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt
fi
if [[ -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt ]]; then
    rm -rf ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt
fi
cellranger count --id="tiny-count-v3" \
 --fastqs="test/shared/cellranger-tiny-fastq/3.0.0/" --sample="tinygex" \
 --transcriptome="test/cellranger_reference/cellranger-tiny-ref/3.0.0"
@@ -68,7 +73,9 @@ fi
if [[ ! -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt.gz ]]; then
    gzip -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt
fi
if [[ -f ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt ]]; then
    rm -rf ${cellrangerpath}-cs/${cellrangerversion}/lib/python/cellranger/barcodes/3M-february-2018.txt
fi
cellranger count --id="tiny-count-v2" \
 --fastqs="test/shared/cellranger-tiny-fastq/1.2.0/" --sample="" --chemistry="threeprime" \
 --transcriptome="test/cellranger_reference/cellranger-tiny-ref/1.2.0"
@@ -80,6 +87,13 @@ fi
if [[ -d input4cellranger_test-10x-v3 ]]; then
    rm -rf input4cellranger_test-10x-v3
fi
# unzip input data
if [[ -f test/shared/cellranger-tiny-fastq/3.0.0/tinygex_S1_L001/*fastq.gz ]]; then
    unpigz -f test/shared/cellranger-tiny-fastq/3.0.0/tinygex_S1_L001/*fastq.gz
fi
if [[ -f test/shared/cellranger-tiny-fastq/3.0.0/tinygex_S1_L002/*fastq.gz ]]; then
    unpigz -f test/shared/cellranger-tiny-fastq/3.0.0/tinygex_S1_L002/*fastq.gz
fi
bash launch_universc.sh --id "test-10x-v3" --technology "10x" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --file "test/shared/cellranger-tiny-fastq/3.0.0/tinygex_S1_L001" \
+10 −9
Original line number Diff line number Diff line
@@ -18,12 +18,10 @@ if [[ ! -f test/cellranger_reference/cellranger-tiny-ref/1.2.0/star/SA ]] && [[
    rsync $(dirname $cellrangerpath)/cellranger-tiny-ref/1.2.0/star/SA test/cellranger_reference/cellranger-tiny-ref/1.2.0/star/SA
fi

unpigz -k test/shared/dropseq-test/SRR1873277*fastq.gz

## test drop-seq data
# unzip input data
if [[ -f test/shared/dropseq-test/*fastq.gz ]]; then
    gunzip -f test/shared/dropseq-test/*fastq.gz
    unpigz -f test/shared/dropseq-test/*fastq.gz
fi
# test manual setup
bash launch_universc.sh -t "nadia" --setup
@@ -35,12 +33,15 @@ fi
if [[ -d input4cellranger_test-dropseq ]]; then
    rm -rf input4cellranger_test-dropseq
fi
if [[ -d test-dropseq ]]; then
    rm -rf test-dropseq
fi

# call on dropseq with files
bash launch_universc.sh --id "test-dropseq" --technology "nadia" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
 --read1 "test/shared/dropseq-test/SRR1873277_Sample1_R1" \
 --read2 "test/shared/dropseq-test/SRR1873277_Sample1_R2" \
 --verbose
 --read2 "test/shared/dropseq-test/SRR1873277_Sample1_R2" 

# reset test data (compress)
 if [[ -f test/shared/dropseq-test/SRR1873277_Sample1_S1_L001_R1_001.fastq ]]; then
+3 −3
Original line number Diff line number Diff line
@@ -33,7 +33,7 @@ if [ -f test/shared/icell8-test/72618_KU812_S2_L002_R2_001.fastq ]; then
    rename -n "s/_S2_L002/_L002/" test/shared/icell8-test/72618_KU812_S2_L002_R2_001.fastq
fi
if [ -d test-icell8-72618-KU812-2-lanes ];then
    rm -rf test-icell8-72618-KU812-2-lanes
    rm -rf test-icell8-72618_KU812-2-lanes
fi
bash launch_universc.sh --id "test-icell8-72618_KU812-2-lanes" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
@@ -47,8 +47,8 @@ fi
if [ -f test/shared/icell8-test/KU812_S1_L001_R2_001.fastq ]; then
    rename "s/_S1_L001/_L001/" test/shared/icell8-test/72618_KU812_S1_L001_R2_001.fastq
fi
if [ -d test-icell8-KU812-1-lane ];then
    rm -rf test-icell8-KU812-1-lane
if [ -d test-icell8-72618_KU812-1-lane ]; then
    rm -rf test-icell8-72618_KU812-1-lane
fi
bash launch_universc.sh --id "test-icell8-72618_KU812-1-lane" --technology "iCell8" \
 --reference "test/cellranger_reference/cellranger-tiny-ref/3.0.0" \
+1 −1

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