Update README.md (ae6740ac) · Commits · github_fork / SHARE Seq AlignmentV2

README.md

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		A barcode translation table indicating the P1.xx used in the assay would be beneficial, when submitting data to GEO.

		# Installation
		This pipeline requires following packages to be properly installed and added to system path: GNU parallel, Bcl2fastq, fastp, zcat, STAR, bowtie2, python3, umi_tools, samtools, picard (2.14.1, newer version may result in error), R, featureCounts, read_distribution.py from RSeQC, bedtools.
		This pipeline requires following packages to be properly installed and added to system path: GNU parallel, Bcl2fastq, fastp, zcat, STAR, bowtie2, python3 (all python code upadted to python3), umi_tools, samtools, picard (2.14.1, newer version may result in error), R, featureCounts, read_distribution.py from RSeQC, bedtools.

		The SHARE-seq-alignment scripts can be directly downloaded from the github website.\
		[https://github.com/masai1116/SHARE-seq-alignmentV2/](https://github.com/masai1116/SHARE-seq-alignmentV2/)