Loading reprocess_deposited_data.md +8 −0 Original line number Diff line number Diff line Loading @@ -16,6 +16,14 @@ FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF: cat SRR19912835_1.fastq | awk '{if(NR%4==1) print "@"$2; else if(NR%4==2) print; else if(NR%4==3) print "+"; else if(NR%4==3) print $0}' | bgzip > speciesmix.ATAC.R1.fastq.gz\ cat SRR19912835_2.fastq | awk '{if(NR%4==1) print "@"$2; else if(NR%4==2) print; else if(NR%4==3) print "+"; else if(NR%4==3) print $0}' | bgzip > speciesmix.ATAC.R2.fastq.gz The new read looks like:\ zless speciesmix.ATAC.R1.fastq.gz | head -4 @A01389:111:H2Y5KDMXY:1:1101:1127:1000_R1.003,R2.032,R3.081,P1.06 GGGCTACACAGAGAAACCCTGTCTCGAAAAACAAACAAAACAAAACAAAA + @A01389:111:H2Y5KDMXY:1:1101:1832:1000_R1.083,R2.003,R3.037,P1.06 3. Move the resulting fastq.gz files into a new directoy (/fakepath/fastqfoler/). 4. Update the rawdir and Project in Share_seqV2_example.sh accordingly:\ Loading Loading
reprocess_deposited_data.md +8 −0 Original line number Diff line number Diff line Loading @@ -16,6 +16,14 @@ FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF: cat SRR19912835_1.fastq | awk '{if(NR%4==1) print "@"$2; else if(NR%4==2) print; else if(NR%4==3) print "+"; else if(NR%4==3) print $0}' | bgzip > speciesmix.ATAC.R1.fastq.gz\ cat SRR19912835_2.fastq | awk '{if(NR%4==1) print "@"$2; else if(NR%4==2) print; else if(NR%4==3) print "+"; else if(NR%4==3) print $0}' | bgzip > speciesmix.ATAC.R2.fastq.gz The new read looks like:\ zless speciesmix.ATAC.R1.fastq.gz | head -4 @A01389:111:H2Y5KDMXY:1:1101:1127:1000_R1.003,R2.032,R3.081,P1.06 GGGCTACACAGAGAAACCCTGTCTCGAAAAACAAACAAAACAAAACAAAA + @A01389:111:H2Y5KDMXY:1:1101:1832:1000_R1.083,R2.003,R3.037,P1.06 3. Move the resulting fastq.gz files into a new directoy (/fakepath/fastqfoler/). 4. Update the rawdir and Project in Share_seqV2_example.sh accordingly:\ Loading
