1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请联
系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
海洋分子生物学实验 Marine Molecular Biology Lab
2.
授课院系
Originating Department
海洋科学与工程系
Department of Ocean Science and Engineering
3.
课程编号
Course Code
OCE318
4.
课程学分 Credit Value
2
5.
课程类别
Course Type
专业选修课 Major Elective Courses
6.
授课学期
Semester
秋季 Fall
7.
授课语言
Teaching Language
中英双语 English & Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
郭静，海洋科学与工程系，创园 9 栋 602，guoj@mail.sustc.edu.cn
Guo Jing, Department of Ocean Science and Engineering,
Chuangyuan 9-602
Tel. 18126401114
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
无 NA
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
10
授课方式
Delivery Method
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
11.
学时数
Credit Hours
64
64
2
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
13.
后续课程、其它学习规划
Courses for which this course
is a pre-requisite
14.
其它要求修读本课程的学系
Cross-listing Dept.
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
本课程目标是为高年级海洋生物学、海洋科学专业学生提供有一点难度的现代海洋分子生物学基本实验技能训练的窗口，
在全面介绍微生物海洋学的基本技术基础上，通过一系列综合性的实验操作，提高学生动手能力，引导并培养学生利用分
子生物学实验手段实现海洋科学研究的基本思维和目的。要完成所有的课程任务，学生必须：
1、完成课前预习,对实验目的、实验原理、实验步骤、注意事项、试剂配方与作用以及实验设计的思路进行理解,鼓励带着
问题上课。
2、按时上课,认真听讲，主动思考，积极回答问题，积极参与课堂讨论，遵守实验室规定，遵守课堂秩序，规范操作；仔
细观察实验过程中每一步骤的实验现象，实事求是地记录并深入分析。通过动手操作能够加深对实验原理与设计思路的理
解，有利于学生创新性思维及独立分析问题解决问题能力的提高。课堂表现、操作表现和出勤率是成绩考核的组成部分。
To complete all course tasks, students must:
1. Complete of pre-class preparation, understanding of the purpose of the experiment, experimental principles,
experimental procedures, precautions, reagent formulation and function, and experimental design ideas, encourage the
class with questions.
2, Attend the class on time, actively think and participate in class discussions, abide by laboratory regulations, observe
classroom order, standardize operations; carefully observe the experimental phenomena at each step of the
experimental process, realistically record and analyze in depth . Through hands-on operation, the understanding of
experimental principles and design ideas can be deepened, which is conducive to students' innovative thinking and the
ability to solve problems independently. Class performance, operational performance and attendance are part of the
performance assessment.
16.
预达学习成果 Learning Outcomes
到课程结束时，学生应能：
1、 了解海洋分子生物学实验技术体系和发展现状，提升深入理解海洋分子生物学理论知识和技术原理的主观愿望，更加
积极地探究现代海洋分子生物学领域相关科学问题并利用现有技术解决实际问题；
2、 能够掌握海洋古菌 SCM1 的培养与生理生化测定。利用流式细胞仪进行海洋微生物样品如 SCMI 的计数与活力分析。
3、 学会海洋酵母菌的分离培养与鉴定等技能。
4、 能够完成海水样品基因组 DNA 提取、PCR 扩增、qPCR 、琼脂糖凝胶电泳等实验操作，并能对实验现象进行观察与
分析。
5、 能够规范使用微量加样器、高压蒸汽消毒器、恒温摇床、超净工作台、高速冷冻离心机、制冰机、PCR 仪、qPCR
仪、水平电泳仪、凝胶成像系统等仪器，熟练无菌操作技术，养成分工协作的团队精神和习惯。
The goal of this course is to provide a high-level marine biology and marine science students with a difficult window of
basic experimental skills training in modern marine molecular biology. Based on a comprehensive introduction to the
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basic techniques of microbial oceanography, through a series of comprehensive The experimental operation enhances
students' practical ability, guides and trains students to use molecular biology experiments to achieve the basic thinking
and purpose of marine scientific research. By the end of the course, students should be able to:
1. Understand the marine molecular biology experimental technology system and development status, enhance the
subjective desire to understand the theoretical knowledge and technical principles of marine molecular biology, and
actively explore relevant scientific issues in the field of modern marine molecular biology and use existing technologies
to solve practical problems. ;
2, Master the cultivation and physiological and biochemical determination of SCM1. Flow cytometry was used to perform
counting and viability analysis of marine microbial samples, such as SCM1.
3. Learn the skills of separation, culture and identification of marine yeast.
4. Master the process of the genomic DNA extraction, PCR amplification, qPCR, agarose gel electrophoresis and other
experimental operations of marine water samples, and can observe and analyze the experimental phenomena.
5. Regulate the use of micro-sampler, high-pressure steam sterilizer, constant temperature shaker, ultra-clean
workbench, high-speed refrigerated centrifuge, ice machine, PCR instrument, qPCR instrument, horizontal
electrophoresis instrument, gel imaging system and other instruments, Proficiency in aseptic technique and teamwork .
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
一、海洋分子生物学实验简介及海水样品过滤(4 学时)
介绍实验室规则制度与课程安排；实验报告要求；实验课考核及评分标准；介绍海水样品采集、保存及运输等实验基础知
识，进行海水样品过滤（不同滤膜孔径样品）。
I. Introduction to marine molecular biology lab course and filtration of seawater sample (4 credit hours)
Introduce the laboratory rules system and curriculum arrangement; experimental report requirements; experimental class
assessment and scoring standards; introduce the basic knowledge of seawater sample collection, preservation and
transportation, and carry out filtration of seawater sample (different filter pore size samples).
二、海水滤膜样品总 DNA 提取（4 学时）
海水和沉积物等海洋环境样品 DNA 的介绍（着重介绍 DNA 提取溶液成分与原理），利用手提法提取海水滤膜样品总
DNA，Nanodrop 测定提取 DNA 浓度和纯度。
2. Total DNA extraction of seawater filter samples (4 credit hours)
Introduction of DNA from marine environmental samples such as seawater and sediments (emphasizing the composition
and principle of DNA extraction solutions), using the portable method to extract total DNA from seawater filtration
samples, and the Nanodrop to determine the concentration and purity of extracted DNA.
三、利用 PCR 扩增海洋古菌基因片段（4 学时）
PCR 原理和多种 PCR 方法及应用介绍，学习引物的设计，利用 PCR 技术扩增已知的海洋古菌基因片段，了解琼脂糖凝
胶电泳的原理；琼脂糖凝胶电泳制备；通过琼脂糖凝胶电泳检测 PCR 产物。
3. Amplification of marine archaeal gene fragments by PCR (4 credit hours)
Introduce PCR principle and various PCR methods and application, learn primer design, using PCR technology to