1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请
联系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
生物医学综合实验 A Biomedical LaboratoryA
2.
授课院系
Originating Department
医学院 School of Medicine
3.
课程编号
Course Code
MED340
4.
课程学分 Credit Value
2
5.
课程类别
Course Type
专业核心课 Major Core Courses
6.
授课学期
Semester
春季/ Spring
7.
授课语言
Teaching Language
英语 English
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
张文勇，医学院，zhangwy@sustech.edu.cn
Zhang Wenyong, School of Medicine, zhangwy@sustech.edu.cn
张婷，医学院，zhangt1@mail.sustech.edu.cn
Zhang Ting, School of Medicine, zhangt1@mail.sustech.edu.cn
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
戴佳佳，医学院，daijj@mail.sustech.edu.cn
Dai Jiajia, School of Medicine, daijj@mail.sustech.edu.cn
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
2
11.
授课方式
Delivery Method
讲授
Lectures
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
学时数
Credit Hours
64
64
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
无 None
13.
后续课程、其它学习规划
Courses for which this
course is a pre-requisite
待定/To be determined
14.
其它要求修读本课程的学系
Cross-listing Dept.
待定/To be determined
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
本课程着重培养学生综合性的实验能力与科研思维。建立糖尿病研究模型综合性实验项目，通过检测不同的
生理指标，观察其相应的生理功能变化，进而理解学习糖尿病的生理病理发展变化进程并掌握一定的综合实
验技能。
This course focuses on cultivating students' comprehensive experimental ability and scientific thinking.
This course establishes an experimental projects of diabetes research model. It explains the functional changes
that accompany the diabetic symdrome by detecting different physiological indicators. It helps student to
understand pathophysiological process in the development of diabetes mellitus and master comprehensive
experimental skills.
16.
预达学习成果 Learning Outcomes
1. 理解学习糖尿病发生发展的生理病理变化过程。
1. To understand pathophysiological change process in the development of diabetes mellitus.
2. 本课程着重培养学生独立思考科学问题的能力、独立设计实验和分析实验结果的能力，为后续学生参加科
技创新项目及生物医学科学研究打下坚实的基础。
2. This course focuses on cultivating students' ability to think scientific problems independently, design
experiments and analyze experimental results independently. Thus, this course lays a solid foundation for the
follow-up projects of science and technology innovation and biological sciences research.
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
3
实验一 绪论
糖尿病相关介绍
实验室安全与垃圾分类
动物实验基本操作技术
Lab 1 Brief introduction
Introduction to diabetes Mellitus.
Laboratory safety and waste classification.
Basic techniques of animal experiment.
实验二 口服葡萄糖耐量试验
观察小鼠糖尿病模型空腹血糖水平
观察胰岛 β 细胞功能和机体对血糖的调节能力
Lab 2 Oral glucose tolerance tests (OGTT)
Introduction for blood glucose levels in diabetes mice.
Observation for the islet β-cells function and the organism 's regulation of blood glucose.
The mice anatomy and sampling.
实验三 糖化血红蛋白的检测
掌握糖化血红蛋白的检测原理
学习糖化血红蛋白的检测的方法
Lab 3 Glycated hemoglobin measurement
Master the principle of HbA1c determination.
To learn the glycosylated hemoglobin (HbA1c) measurements.
实验四 基因型鉴定
了解小鼠基因型鉴定的各种方法
学习 PCR的方法进行基因型鉴定
Lab 4 Genotype Identification
Be familiar with the methods of mouse genotyping.
Learn to do genotyping by PCR.
实验五 皮肤切创愈合
掌握小鼠皮肤切创模型的建立
观察比较糖尿病小鼠的伤口愈合不良
Lab 5 Skin Wound Healing
Master the method to create murine model of wound healing.
To observe impaired wound healing of diabetic mice model.
实验六 脑与脊髓的取材
学习小鼠灌注、组织固定及组织脱水技术
学习小鼠的脑与脊髓的取材
Lab 6 Isolation of Brain and Spinal Cord
To learn the techniques of mouse perfusion, tissue fixation and tissue dehydration.
To learn the techniques of dissecting brain out of the skull and isolation of spinal cord.
实验七 脑与脊髓的切片
学习脑与脊髓的包埋与冰冻切片技术
4
学习鉴别脊髓的腰骶膨大区与脑片的海马区
Lab 7 Sectioning of Brain and Spinal Cord
Learn the techniques of brain and spinal cord embedding and frozen sectioning.
To identify the lumbosacral enlargement region of spinal cord and hippocampus of brain.
实验八 自选课题开题汇报
Lab 8 Thesis proposal of optional subjects
实验九 免疫荧光染色
学习免疫荧光染色的原理
观察比较实验组小鼠与对照组小鼠脊髓的病理变化
Lab 9 Immunofluorescent Staining
To learn about the principle of immunofluorescent staining
To observe histopathological changes in the spinal cord of the DB mouse
实验十 HE 染色与 TUNEL 凋亡检测
学习 HE染色与 TUNEL 凋亡检测的原理
观察比较实验组与海马区域病理变化的区别
Lab 10 HE Staining and TUNEL Assay
Master the principle of HE staining and TUNEL assay
To observe histopathological changes in hippocampus of the DB mouse under a microscope.
实验十一 流式免疫学检测
了解流式细胞技术
学习运用流式细胞技术对糖尿病模型进行免疫学检测
Lab 11 Immunological detection by flow cytometry
Understanding flow cytometry features of single Cells and many features
To learn the Immunological detection by flow cytometry in diabetic mice model.
实验十二 糖尿病眼病检测观察
视网膜取材与铺片观察
观察比较实验组与对照组之间的视网膜微血管病变
Lab 12 Detection of diabetic retinopathy
Retinal sampling and wholemount in mice.
To observe the retinal neovascularization differences between DB mice and control mice.
实验十三 组织蛋白提取
学习制备组织匀浆及从肝肺肾组织提取蛋白
学习 BCA方法测定组织蛋白浓度的原理
Lab 13 Harvest of Tissue Proteins
To process tissue homogenate and to extract proteins from livers , lungs and kidneys.
To understand the principle of BCA protein assay and to determine the concentration of protein samples.
实验十四 SOD 的测定
学习 ELISA的原理与实验操作
测定肝肺肾组织中的氧化应激情况
Lab 14 SOD Determination
To learn about the principle and procedure of ELISA assay.
5
To determine the levels of SOD in the livers , lungs and kidneys protein samples.
实验十五 热痛阈值的检测
观察不同实验组小鼠在加热过程中的热痛阈值反应
理解热痛阈值的定义
Lab 15 Determination of Thermal Nociceptive Thresholds
To observe nociceptive response to heat among different groups mice.
Master the identifcation of nociceptive response to thermal stimulation.
实验十六 总结汇报
Lab 16 Review presentation
Section
Time
Teaching Contents
1
4
绪论，动物实验基本操作
Brief introduction, Basic techniques of animal experiment
2
4
口服葡萄糖耐量试验
Oral glucose tolerance tests (OGTT)
3
4
糖化血红蛋白的检测
Glycated hemoglobin measurement
4
4
基因型鉴定
Genotype identification
5
4
皮肤切创愈合实验
Skin Wound Healing
6
4
脑与脊髓取材
Isolation of Brain and Spinal Cord
7
4
脑与脊髓切片
Sectioning of Brain and Spinal Cord
8
4
自选课题之开题报告
Thesis proposal of optional subjects
9
4
免疫荧光染色
Immunofluorescent Staining
10
4
HE 染色与凋亡检测
HE Staining and Tunel Assay
11
4
糖尿病眼病检测观察
Detection of diabetic retinopathy
12
4
流式免疫学检测
Immunological detection by flow cytometry
13
4
热痛反应阈值检测
Thermal nociceptive thresholds (TNTs)
14
4
组织蛋白提取
Harvest of Tissue Proteins
15
4
SOD 检测
SOD Determination
16
4
总结汇报
Review presentation
18.
教材及其它参考资料 Textbook and Supplementary Readings
6
Ian A.Cree. Cancer Cell Culture Methods and Protocols. 2nd edition. Hummana Press. 2011
扈瑞平, 郑明霞. 生物医学综合实验指导. 北京大学医学出版社. 2016
课程评估 ASSESSMENT
19.
评估形式
Type of
Assessment
评估时间
Time
占考试总成绩百分比
% of final
score
违纪处罚
Penalty
备注
Notes
出勤 Attendance
10
课堂表现
Class
Performance
20
小测验
Quiz
课程项目 Projects
平时作业
Assignments
50
期中考试
Mid-Term Test
期末考试
Final Exam
期末报告
Final
Presentation
20
其它（可根据需要
改写以上评估方
式）
Others (The
above may be
modified as
necessary)
20.
记分方式 GRADING SYSTEM
A. 十三级等级制 Letter Grading
B. 二级记分制（通过/不通过） Pass/Fail Grading
课程审批 REVIEW AND APPROVAL
21.
本课程设置已经过以下责任人/委员会审议通过
This Course has been approved by the following person or committee of authority
本课程已经医学院教务处主任张文勇教授审核通过。