1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请联
系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
生物医学综合实验，Biomedical Laboratory
2.
授课院系
Originating Department
医学院，School of Medicine
3.
课程编号
Course Code
MED308
4.
课程学分 Credit Value
1
5.
课程类别
Course Type
专业核心课 Major Core Courses
6.
授课学期
Semester
春 Spring
7.
授课语言
Teaching Language
中 Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
张文勇，教授，南方科技大学医学院
zhangwy@sustc.edu.cn
0755-88018026
Zhang Wenyong, Professor, School of Medicine, Southern University of Science and
Technology
Email: zhangwy@sustc.edu.cn
0755-88018026
董金堂，教授，南方科技大学医学院
dongjt@sustc.edu.cn
0755-88018032
DONG Jintang, Professor, School of Medicine, Southern University of Science and
Technology
Email: dongjt@sustc.edu.cn
0755-88018032
卢奕，副教授，南方科技大学医学院
luy3@sustc.edu.cn
0755-88015570
Lu Yi, Associate Professor, School of Medicine, Southern University of Science and
Technology
Email: luy3@sustc.edu.cn
0755-88015570
2
刘泉，副教授，南方科技大学医学院
liuq3@sustc.edu.cn
0755-88018055
Liu Quan, Associate Professor, School of Medicine, Southern University of Science and
Technology
Email: liuq3@sustc.edu.cn
0755-88018055
陈国安，副教授，南方科技大学医学院
cheng@sustc.edu.cn
0755-88018042
Chen Guoan, Associate Professor, School of Medicine, Southern University of Science
and Technology
Email: cheng@sustc.edu.cn
0755-88018042
张志潜，研究助理教授，南方科技大学医学院
zhangzq@sustc.edu.cn
0755-88018032
ZHANG Zhiqian, Research Assistant Professor, School of Medicine, Southern University
of Science and Technology
Email: zhangzq@sustc.edu.cn
0755-88018032
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
无 NA
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
授课方式
Delivery Method
讲授
Lectures
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
11.
学时数
Credit Hours
0
0
32
0
32
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
无 None
13.
后续课程、其它学习规划
Courses for which this course
is a pre-requisite
无 None
14.
其它要求修读本课程的学系
Cross-listing Dept.
无 None
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
3
本实验课程设置两个实验项目，建立小鼠糖尿病模型和肿瘤研究模型。
This lab course has set up two independent experimental projects: diabetic mouse model and tumor research model.
1.小鼠糖尿病模型实验是一个综合性的实验，通过检测不同的生理指标，观察其相应的生理功能变化，进而理解学习糖尿
病的生理病理发展变化进程并掌握一定的综合实验技能。For mouse diabetic model: a comprehensive laboratory involving
physiology and pathophysiology. It explains the functional changes that accompany the diabetic syndrome by detecting
different physiological indicators. It helps student to understand pathophysiological process in the development of
diabetes mellitus and master comprehensive experimental skills.
2.肿瘤模型中：建立与小鼠皮下移植肿瘤模型，使学生加深对肿瘤生长的认识，探讨肿瘤基因及抑癌基因的作用功能；设
计体外检测，检测抗肿瘤药物的作用效果。For tumor research model: the establishment of mouse subcutaneous
injection tumor model can be helpful for student to deep understand tumor growth and explore the function of tumor
genes and tumor suppressor genes. This model also involves in vitro detection assays to test the effect of anti-tumor
drugs.
16.
预达学习成果 Learning Outcomes
1. 掌握小鼠糖尿病模型及肿瘤研究模型这两种常用动物模型。To understand the common animal models of diabetes
research model and tumor research model.
2. 理解学习糖尿病及肿瘤的发生发展的生理病理变化过程。To understand pathophysiological change process in the
development of diabetes mellitus and tumor.
3. 本课程着重培养学生独立思考科学问题的能力、独立设计实验和分析实验结果的能力，为后续学生参加科技创新项目及
生物医学科学研究打下坚实的基础。This course focuses on cultivating students' ability to think scientific problems
independently, design experiments and analyze experimental results independently. Thus, this course lays a solid
foundation for the follow-up projects of science and technology innovation and biological sciences research.
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
SECTION A: Tumor Model
实验一 癌细胞培养的基本技术
掌握细胞培养的无菌操作和细胞计数
熟悉不同种类的癌细胞的常规培养方法
掌握癌细胞的传代培养方法以用于其功能性实验检测。
Lab 1 Essential techniques of cancer cell culture
Fundamentals in aseptic technique and Hemacytometer counting.
Be Familiar with routine maintenance of different cancer cells.
Learn to seed cancer cell for functional assays.
实验二 小鼠皮下注射肿瘤模型
掌握小鼠皮下移植肿瘤的操作技术
观察肿瘤生长曲线
Lab 2 Mouse subcutaneous injection tumor model
Master the operation technique of subcutaneous tumor in mice.
4
Observation tumor growth curve.
实验三 癌细胞增殖分析
观察药物治疗后癌细胞生长的差异
体外检测评价抗癌药物的作用效果
Lab 3 Cancer cell proliferation assay
Observation the difference of cancer cell growth after drug treatment.
Evaluate anti-cancer drug’s function in in vitro assay.
实验四 癌细胞凋亡分析
应用 Annexin V 染色和选择性碘化丙啶（PI）检测抗癌药物处理后的癌细胞凋亡情况
体外检测评价抗癌药物的作用效果
Lab 4 Cancer cell apoptosis assay
Detection cancer cell apoptosis using annexin V staining and optional propidium iodide (PI)
Evaluate anti-cancer drug’s function in in vitro assay.
实验五 癌细胞迁移实验
量化细胞移动，观察癌细胞迁移后贴壁生长的情况
评价趋化因子的作用
Lab 5 Cancer cell transwell Assay
Quantify cell movement and measure migration of adherent cells.
Evaluate chemoattractant properties.
实验六 RNA 提取和实时荧光定量 PCR
测定靶基因的表达
掌握 RNA 提取和基因表达数据分析的实验技巧
Lab 6 RNA extraction and Real-time qPCR
Determine the expression of target genes.
Acquire the experiment skills of RNA extraction and gene expression data analysis.
SECTION B: Diabetes Model
实验一 口服葡萄糖耐量试验和 β-羟丁酸检测
观察小鼠糖尿病模型的酮酸与空腹血糖水平
观察胰岛 β 细胞功能和机体对血糖的调节能力
Lab 1 Oral glucose tolerance tests (OGTT) and β-HB measurements
Introduction for keto acid and blood glucose levels in diabetes mice.
Observation for the islet β-cells function and the organism 's regulation of blood glucose.
实验二 皮肤切创愈合实验和糖化血红蛋白的检测
掌握小鼠皮肤切创愈合模型
观察糖尿病小鼠的皮肤愈合问题
5
掌握 Elisa 检测糖化血红蛋白的方法
Lab 2 Skin Wound Healing and glycosylated hemoglobin (HbA1c) measurements
Master murine model of wound healing in mice.
To observe impaired wound healing of diabetic mice model.
To learn the glycosylated hemoglobin (HbA1c) measurements with Elisa.
实验三 SOD 和 MDA 检测，胰岛素（C 肽）释放试验
掌握化学发光法检测的方法与原理
观察糖尿病小鼠的氧化应激反应
Lab 3 Detection of SOD and MDA, Insulin release test (IRT)
Master the method and principle of chemiluminescence.
Identified oxidative stress reaction in diabetes mice.
实验四 基因型鉴定
掌握基因型的鉴定方法与原理
对照组、STZ-1 组、STZ-2 组、Lepdb/db 组小鼠，鉴定不同组别的基因型
Lab 4 Genotype identification
Master the method and principle of genotype identification.
Identified genotypes in control group, STZ-1 group, STZ-2 group and Lepdb/db group.
实验五 热痛反应阈值检测
掌握热痛反应阈值的定义
观察糖尿病神经病变的痛觉过敏现象
Lab 5 Thermal nociceptive thresholds (TNTs)
Master the definition of thermal nociceptive thresholds.
To observe hyperalgesia phenomenon for painful diabetic neuropathy.
实验六 质谱流式进行免疫学检测
了解质谱流式细胞技术
学习运用质谱流式细胞技术对糖尿病模型进行免疫学检测
Lab 6 Immunological detection by mass cytometry
Understanding mass cytometry features of single Cells and many features
To learn the Immunological detection by mass cytometry in diabetic mice model.
实验七 质谱流式细胞技术的数据分析
学习质谱流式数据在线分析
了解质谱流式数据分析软件： Cytobank
Lab 7 Introduction of mass cytometry data analysis
To learn data online analysis of mass cytometry.
Understanding mass cytometry data analysis software: Cytobank.
Section
Experiment
Time
1
Essential techniques of cancer cell culture
2h
6
癌细胞培养的基本技术
2
Cancer cell proliferation assay
癌细胞增殖分析
2h
3
Mouse subcutaneous injection tumour model
小鼠皮下注射肿瘤模型
2h
4
Cancer cell apoptosis assay
癌细胞凋亡分析
2h
5
Cancer cell transwell assay
癌细胞迁移实验
2h
6
Cancer cell RNA extraction
癌细胞 RNA 提取
2h
7
Mouse tumour isolation and histological analysis
小鼠肿瘤分离和组织学分析
2h
8
Real-time PCR
实时荧光定量 PCR
2h
9
Fasting blood glucose and oral glucose tolerance
test (OGTT), β-HB measurements
空腹血糖与口服葡萄糖耐量试验，β-羟丁酸检测
2h
10
Skin wound healing and glycosylated hemoglobin
(HbA1c) measurements
皮肤切创愈合实验，糖化血红蛋白的检测
2h
11
Detection of SOD and MDA, Insulin release
test(IRT)
SOD 和 MDA 检测，胰岛素（C 肽）释放试验
2h
12
Genotype identification
基因型鉴定
2h
13
Determination of thermal nociceptive thresholds
热痛反应阈值检测
2h
14
Immunological detection by mass spectrometer
质谱流式细胞技术进行免疫学检测
2h
15
Introduction of mass cytometry data analysis
质谱流式细胞的数据分析
2h
16
Experiment summary and data analysis
实验总结和数据分析
2h
18.
教材及其它参考资料 Textbook and Supplementary Readings
Ian A.Cree. Cancer Cell Culture Methods and Protocols. 2nd edition. Hummana Press. 2011
扈瑞平, 郑明霞. 生物医学综合实验指导. 北京大学医学出版社. 20166.
课程评估 ASSESSMENT
19.
评估形式
Type of
Assessment
评估时间
Time
占考试总成绩百分比
% of final
score
违纪处罚
Penalty
备注
Notes
7
出勤 Attendance
10
课堂表现
Class
Performance
20
小测验
Quiz
课程项目 Projects
平时作业
Assignments
70
期中考试
Mid-Term Test
期末考试
Final Exam
期末报告
Final
Presentation
其它（可根据需要
改写以上评估方
式）
Others (The
above may be
modified as
necessary)
20.
记分方式 GRADING SYSTEM
A. 十三级等级制 Letter Grading
B. 二级记分制（通过/不通过） Pass/Fail Grading
课程审批 REVIEW AND APPROVAL
21.
本课程设置已经过以下责任人/委员会审议通过
This Course has been approved by the following person or committee of authority
