课程大纲
COURSE SYLLABUS
1.
课程代码/名称
Course Code/Title
****/高级显微镜：基础与运用
Advanced Microscope: Foundation and Application
2.
课程性质
Compulsory/Elective
必修
Compulsory
3.
课程学分/学时
Course Credit/Hours
3 学分/48 学时
3 Points/48 Periods
4.
授课语言
Teaching Language
English
5.
授课教师
Instructor(s)
Dayong Jin
6.
是否面向本科生开放
Open to undergraduates
or not
Not
7.
先修要求
Pre-requisites
（ 如 面 向 本 科 生 开 放 ， 请 注 明 区 分 内 容 。 If the course is open to
undergraduates, please indicate the difference.）
光学、细胞生物学、医学影像技术学、光电检测
8.
教学目标
Course Objectives
了解显微成像发展历史与显微镜分类及应用特征，熟悉当前主流显微成像技术最新发展动态，掌握各
类显微成像技术的物理原理、系统构成及临床和生物医学应用，具备对各类成像技术后续深入研究的
能力。
（如 面 向 本 科 生开 放 ， 请 注 明 区分 内 容 。 If the course is open to undergraduates, please indicate the
difference.）
9.
教学方法
Teaching Methods
课堂讲授+主题报告+辅导答疑
（如面向本科生 开放， 请注明 区分内 容。 If the course is open to undergraduates, please indicate the
difference.）
10.
教学内容
Course Contents
（如面向本科生开放，请注明区分内容。 If the course is open to undergraduates, please indicate the
difference.）
Section 1
Overview
Section 2
Microscopy Basics and History
Section 3
Basics of Light - Light-matter interactions
Section 4
Light source and lasers
Section 5
Pulsed Laser & Femtosecond Lasers
Section 6
Laser tissue interactions and laser safety
Section 7
Optical Filters (spectrum)
Section 8
Detectors
Section 9
Cameras
Section 10
Fibers
Section 11
Lens and Objectives
Section 12
Fluorescent Dyes and Stains
Section 13
Fluorescent Proteins
Section 14
Advanced Fluorescent Probes
Section 15
Fluorescence microscopy (Part I)
Section 16
Fluorescence (Stereo) microscopy (Part II)
Section 17
Time-gated luminescence microscopy
Section 18
TIRF microscopy
Section 19
Contrast in Optical Microscopy
Section 20
Scanning Microscopy & Flow Cytometry
Section 21
Confocal Microscopy – PSF engineering
Section 22
Confocal Microscopy – Scanning and Spin Disc
Section 23
Two photon microscopy
Section 24
Super Resolution microscopy
…………
课程考核
Course Assessment
平时作业 30%+课程报告 30%+结业答辩 40%
11.
（
○
1 考核形式 Form of examination；
○
2 .分数构成 grading policy；
○
3 如面向本科生开放，请注明区分内容。
If the course is open to undergraduates, please indicate the difference.）
48 Lectures over 8 weeks: The class consists primarily of lectures and lecture discussions with
mini tutorials and mini guest lectures as necessary.
Reading & attendance, and discussion (30%)
Seminar (30% + 10%): individual + group performance score
There is an end of term quiz (30%) – week 8 (27 Dec)
Final Grade: reading & attendance + seminar + end of term quiz
Evaluation Instrument
– questions (prepare you better for the course and your postgraduate research)
- the lecture content and the lecturer (help this lecture better)
教材及其它参考资料
Textbook and Supplementary Readings
12.
《工程光学》、《声学显微镜与超分辨率成像理论及应用》、《活细胞成像》、《细胞生物学荧光技
术原理和应用》
