课程大纲

COURSE SYLLABUS

课程代码

名称

Course Code/Title

高级微生物学

/ Advanced Topics in Microbiology

课程性质

Compulsory/Elective

Elective

课程学分

学时

Course Credit/Hours

3 credits /48 hours

授课语言

Teaching Language

English and Chinese

授课教师

Instructor(s)

Tao Dong

是否面向本科生开放

Open to undergraduates

or not

先修要求

Pre-requisites

（如面向本科生开放，请注明区分内容。If the course is open to undergraduates, please

indicate the difference.）

Microbiology, Biochemistry

微生物学 / 生物化学

教学目标

Course Objectives

（如面向本科生开放，请注明区分内容。 If the course is open to undergraduates, please indicate the difference.）

The main objective is to introduce important topics in microbiology to graduate students. These topics include quorum sensing,

horizontal gene transfer, antibiotic resistance, and protein secretion systems. These topics are modular and are not

interdependent. These modules could also be flexibly rotated in different terms to customize toward the student’s needs and

interests. In addition, students will also be trained in writing and presentation kills in the context of microbiology common

practice by presenting and critically evaluating research advances from the research literature and through written assignments.

本课程的主要目的是向研究生介绍微生物学的一些重要的知识和前沿进展。内容包括群体感应、水平基因转移、抗生

素耐药性和蛋白质分泌系统。不同内容将以模块化的形式进行，彼此之间是独立的。这些模块也可以根据学生的需求

和兴趣进行灵活的变化。此外，学生还将通过报告展示、批判性地评估文献和书面作业的形式，进行微生物学有关的

写作和报告展示能力的训练。

教学方法

Teaching Methods

（如面向本科生开放，请注明区分内容。 If the course is open to undergraduates, please indicate the difference.）

The course will be structured in a hybrid format with 1/2 in-class lectures, 1/4 student presentations, and 1/4 group discussions.

Al required lecture materials and assignments will be available to students. Communication with the students will be primarily

in person in class and email response after class.

课程将采用混合形式，分别为 1/2 的课堂讲授、1/4 的学生报告和 1/4 的小组讨论。所有必需的课程材料和作业都将提

供给学生。与学生的交流方式主要为课堂上的面对面交流和课后的邮件回复。

10.

教学内容

Course Contents

（如面向本科生开放，请注明区分内容。 If the course is open to undergraduates, please indicate the difference.）

Section 1

Course information (requirement and expectation) and topic overview

• Prerequisites, grading, presentation and writing examples, and course

introduction

课程信息（要求和预期）以及话题概述

• 选课条件、评分、报告展示和写作范例以及课程介绍

Section 2

Tools and skills in Microbiology

• Bacterial growth in monoculture and polyculture

• Genetic screening

• Transformation and conjugation

• Power of Reporters

微生物学的工具和技能

• 单一培养和混合培养中的细菌生长

• 遗传筛查

• 转移和接合

• 报告基因的作用

Section 3

Protein secretion systems

• Overview of secretion systems

• Type 3 secretion

• Type 6 secretion

蛋白分泌系统

• 分泌系统概述

• III型分泌系统

• VI型分泌系统

Section 4

Horizontal gene transfer

• Mechanism of HGT

• Impact of HGT

水平基因转移

• 水平基因转移的机制

• 水平基因转移的影响

Section 5

Stress response and sigma factors

• Overview of gene regulation

• Importance of stress response

• Types of sigma factors

胁迫反应和sigma 因子

• 基因调控概述

• 胁迫反应的重要性

• sigma 因子的类型

Section 6

Quorum sensing

• History and types of quorum sensing

• Regulatory network

群体感应系统

• 群体感应系统的历史和类型

• 调控网络

Section 7

Signal transduction

• Bacterial intracellular signal molecules

• Magic spot

• c-di-GMP

信号转导

• 细菌胞内信号分子

• 环鸟苷二磷酸

Section 8

Antimicrobial resistance

• Mechanism of resistance

• Discovery of new drugs

抗生素耐药

• 耐药机制

• 新药物的发现

Section 9

Phage and virus

• Phage infection and CRISPR

• Phage application

噬菌体和病毒

• 噬菌体感染和CRISPR

• 噬菌体应用

Section 10

Microbiome

• Host microbiome

• Environmental microbiome

微生物组

• 宿主微生物组

• 环境微生物组

Section 11

Gut-Brain axis

• Gut microbiome and immunity

• Pain and infection

肠-脑轴

• 肠道微生物群和免疫

• 疼痛和感染

Section 12

Review of course materials

• Review course topics

• Question and Answer

课程内容回顾

• 课程回顾

• 问答

11.

课程考核

Course Assessment

（

○

1 考核形式 Form of examination；

○

2 .分数构成 grading policy；

○

3 如面向本科生开放，请注明区分内容。 If the

course is open to undergraduates, please indicate the difference.）

Evaluation will be based on the following three sections

1: Participation 20%

2: Presentation 40%

3: Written report 40%

评估将基于以下三个部分：

1：出勤 20%

2：报告展示 40%

3：书面报告 40%

12.

教材及其它参考资料

Textbook and Supplementary Readings