课程大纲
COURSE SYLLABUS
1.
课程代码/名称
Course Code/Title
Introduction to Cryo-EM
2.
课程性质
Compulsory/Elective
Elective
3.
课程学分/学时
Course Credit/Hours
1credits 16 hours
4.
授课语言
Teaching Language
Chinese/English
5.
授课教师
Instructor(s)
Chuang Liu 刘闯
6.
先修要求
Pre-requisites
N/A
7.
教学目标
Course Objectives
The students will have a comprehensive understanding of Cryo-EM, understanding the basic principles and
procedures. When having a new sample at hand, they will know how and where to get started.
8.
教学方法
Teaching Methods
Teaching in classroom and practicing data processing with students’ own laptops
9.
教学内容
Course Contents
Section 1
Principles of electron optics
Section 2
Structure of Electron microscope
Section 3
Formation of image
Section 4
Sample preparation methods
Section 5
Image processing
Section 6
3D reconstruction and result interpolation
Section 7
Data processing practice
Section 8
Data processing practice
Section 9
Section 10
…………
10.
课程考核
Course Assessment
请再此注明：①考查/考试；②分数构成。
Course with the requirement to submit a term paper
Scores will be composed by requirement paper, attendance.
11.
教材及其它参考资料
Textbook and Supplementary Readings
冷冻电镜相关书籍：
《Three-Dimensional Electron Microscopy of Macromolecular Assemblies》
by Joachim Frank
冷冻电镜基本原理书籍：
《Diffraction Physics》Third Revised Edition by John M.Cowley
《电子衍射物理教程》 by 王蓉
《电子晶体学与图像处理》by 李方华
《薄晶体电子显微学》by P. Hirsch
视频：
冷冻电子显微镜（Cryo-EM）入门 by 加州理工学院 Grant J.Jensen
链接：https://www.coursera.org/learn/cryo-em
MRC Lectures:
链接：ftp://ftp.mrc-lmb.cam.ac.uk/pub/scheres/EM-course
Advances in Cryo-EM related book
Cryo-EM Part A-C & Recent advances in cryo-EM (Methods in Enzymology)
链接：http://www.sciencedirect.com/science/bookseries/00766879/481
http://www.sciencedirect.com/science/bookseries/00766879/482
http://www.sciencedirect.com/science/bookseries/00766879/483
http://www.sciencedirect.com/science/bookseries/00766879/579
Reviews of Cryo-EM：
"A primer to single-particle cryo-electron microscopy."
https://www.ncbi.nlm.nih.gov/pubmed/25910204
"Structural analysis of macromolecular assemblies by electron
microscopy." https://www.ncbi.nlm.nih.gov/pubmed/21919528
Structural Analysis of Protein Complexes by Cryo Electron Microscopy
https://link.springer.com/protocol/10.1007%2F978-1-4939-7033-9_28
