1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请
联系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
基因组学 Genomics
2.
授课院系
Originating Department
生物系
Department of Biology
3.
课程编号
Course Code
BIO350
4.
课程学分 Credit Value
3
5.
课程类别
Course Type
专业选修课 Major Elective Courses
6.
授课学期
Semester
春季 Spring
7.
授课语言
Teaching Language
中英双语 English & Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
郑梅珍、生物系、电话 15800474108
Meizhen Zheng, Department of Biology, Phone: 15800474108
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
待公布
To be determined
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
无
None
2
11.
授课方式
Delivery Method
讲授
Lectures
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
学时数
Credit Hours
48
48
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
BIO103 生物学原理和 BIO301 遗传学
Principles of Biology and Genetics
13.
后续课程、其它学习规划
Courses for which this
course is a pre-requisite
基因组学是一门具有很强的理论性和实用性的交叉学科, 对深化与开拓生命科学新的研
究方向具有重大的意义。基因组学将引领 21 世纪现代生命科学。从源头上揭示生命的奥
秘，必须从结构基因组学和功能基因组入手。本课程为生物系专业选修课，具备基础的生
化知识、遗传学知识的同学均可选修本课程。
Genomics is an interdisciplinary subject with strong theoretical and practicality, which is
of critical meaning to deepen and open up new research directions in life sciences.
Genomics would lead the development of modern life sciences in the 21
st
century. To
uncover the mysteries of life from the origin, we must start with structural genomics and
functional genomics. This course is a Major Elective Course in biology, and it is suitable
for the students who have basic knowledge of biochemistry or genetics.
14.
其它要求修读本课程的学系
Cross-listing Dept.
无 None
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
基因组学从结构基因组学和功能基因组入手，从源头上揭示生命的奥秘。该课程的教学目标：1. 了解基因组学的发展历
史； 2. 了解全基因组测序的策略、路线和方法；3. 熟悉基因组的结构及基因功能元件，以及功能元件的鉴定技术；4. 熟
悉基因组的表观遗传与基因调控；5. 熟悉转录组及检测技术；6. 了解基因组学与转录组学的关系。通过结合研究实例，讲
解基因组学的研究思路、原理、技术与方法，深入理解基因组学领域的最新进展及其对生命科学各领域的影响，进而从本
质上理解生命的起源、变异与进化。
Genomics starts with structural genomics and functional genomics to uncover the mysteries of life from the origin. The
aim is: (1) To understand the development history of genomics; (2) To understand the strategy, route, and method of
whole genome sequencing; (3) To understand the structure of the genome, the functional elements and the identification
techniques; (4) To understand the epigenetics and gene regulation; (5) To understand the transcriptome and associated
techniques; (6) To understand the relationship between Genomics and transcriptomics. By using examples to explain
research ideas, principles, techniques and methods of genomics, finally, in-depth understanding and familiarity with the
latest developments in the field of genomics and their impact on various fields of life sciences. Furthermore, to
understand the origin, mutation and evolution of life fundamentally.
16.
预达学习成果 Learning Outcomes
学生会对基因组学有一个系统的了解。 这主要包括：基因组的结构和功能；基因组学的研究方法；研究基因组结构与功
能的技术手段与方法等。基因组学对理解当代生命科学中诸多极具突破性的技术革新和方法革新的理论基础有重要的指导
性意义。
Students will have a systematic understanding of genomics. This mainly includes: the structure and function of the
genome; the research methods of genomics; the technical strategies and methods to study the structure and function of
the genome, etc. This would have important guiding significance for understanding the theoretical basis of many
breakthrough technological innovations in contemporary life sciences.
3
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
Lecture No.
讲座题目
Lecture Topic
学时
Hours
具体讲座内容
Lecture content
1
基因组学总体介绍
Introduction of
Genomics
2 学时
2 hours
基因组学的概念 (The concept of Genomics)
基因组学的发展史 (The history of genomics)
2
人类基因组计划
Introduction of the
Human Genome
Project (HGP)
2 学时
2 hours
什么是人类基因组计划
What is the Human Genome Project (HGP)
人类基因组计划的研究历史 (The research history of
HGP)
人类基因组计划的研究领域 (The research field of
HGP)
3
Homework 1
二代基因组测序技术
Second-generation
sequencing
techniques
2 学时
2 hours
二代测序技术的种类
二代测序技术的原理
二代测序技术的应用
The type of second-generation sequencing methods
The principle of second-generation sequencing
methods
The application of second-generation sequencing
methods
4
三代基因组测序技术
Third-generation
sequencing
techniques
2 学时
2 hours
Nanopore
PacBio
Linked Read Sequencing – 10X Genomics
Chromium Technology
5
基因组结构
The structure of
genome
2 学时
2 hours
基因组的结构与大小
原核生物的基因组结构
真核生物的基因组结构
The structure and size of genome
The genome structure of prokaryote
The genome structure of eukaryote
4
6
基因组功能元件
The functional
element of genome
2 学时
2 hours
基因组功能元件的定义
基因组功能元件的分类
The definition of genome functional elements
The classification of functional elements
7
基因组功能元件注释
的进展阶段-1
The stage of the
functional element
annotation for
genome
2 学时
2 hours
DNA 元件百科全书阶段 1 (Encode 1)
DNA 元件百科全书阶段 2 (Encode 2)
8
基因组功能元件注释
的进展阶段-2
The stage of the
functional element
annotation for
genome
2 学时
2 hours
DNA 元件百科全书阶段 3 (Encode 3)
DNA 元件百科全书阶段 4 (Encode 4)
9
染色质状态注释
The annotation of
chromatin states
2 学时
2 hours
核小体 (Nucleosome)
常染色质 (Euchromatin)
异染色质(Heterochromatin)
染色质状态(Chromatin states)
10
染色质状态检测技术
The detecting
techniques for
chromatin states
DNase-seq
ATAC-seq
11
Homework 2
增强子、启动子、绝
缘子注释与检测技术
The annotation and
detection method for
enhancer, promoter,
and insulator
2 学时
2 hours
增强子 (Enhancer)
启动子 (Promoter)
绝缘子 (Insulator)
全基因组功能性调控元件的鉴定 (Genome-wide
identification of regulatory elements)
ChIP-seq
STARR-seq
12
基因组远程交互
The long-range
interactions of 3D
Genome
2 学时
2 hours
三维基因组学概念介绍(The concept of 3D Genome)
三维基因组学发展历史 (The history of 3D Genome)
13
基因组交互技术
2 学时
3C
5
The interaction
techniques for 3D
Genome
2 hours
4C
Hi-C
14
Homework 3
特异因子介导的基因
组远程交互技术
The interaction
techniques for the
specific factor
meditated long-range
interaction for 3D
Genome
2 学时
2 hours
ChIA-PET
Long-read ChIA-PET
15
新一代基因组远程交
互技术
The new generation
of 3D Genome
technology
2 学时
2 hours
SPRITE
ChIA-Drop
16
基因组表观遗传介绍-
1
The introduction of
Genomics &
epigenetics
2 学时
2 hours
表观遗传的定义 (The definition of epigenetics )
DNA 甲基化及其检测技术 (DNA methylation and its
detection techniques)
17
基因组表观遗传介绍-
2
The introduction of
Genomics &
epigenetics
2 学时
2 hours
染色质结构与表观标记
Chromatin structure and histone marks
18
转录组
Transcriptome
2 学时
2 hours
转录组定义(The definition of transcriptome)
转录组对象(The object of transcriptome)
转录组优势(The advantage of transcriptome)
19
Homework 4
转录组相关技术
The techniques of
transcriptome
2 学时
2 hours
基因芯片 (Microarray)
mRNA 测序 (mRNA sequencing)
Total RNA 测序 (Total RNA sequencing)
20
单细胞转录组
Single-cell
transcriptome
2 学时
2 hours
单细胞转录组定义
单细胞转录组对象
单细胞转录组优势
The definition of single-cell transcriptome
The object of single-cell transcriptome
6
The advantage of single-cell transcriptome
21
单细胞转录组相关技
术
The techniques of
single-cell
transcriptome
2 学时
2 hours
10x genomics
SMART-seq2
22
Homework 5
基因组与转录组
Genome and
transcriptome
2 学时
2 hours
基因组到转录组
基因组编码区序列变异影响转录组的表达谱
基因组非编码区序列变异影响转录组的表达谱
From genome to transcriptome
Sequence variations in coding region affect gene
expression
Sequence variations in non-coding region affect
gene expression
23
基因组学与肿瘤研究
Genomics and
Cancer research
2 学时
2 hours
肿瘤的基因组图谱分析
The genomic landscape of cancer
24
综述基因组学的发展
与应用
Write a review about
the application and
development of
Genomics
2 学时
2 hours
18.
教材及其它参考资料 Textbook and Supplementary Readings
参考教材：
高等教育出版社出版的《基因组学》第 3 版或者第 4 版，杨金水编著。
Introduction to Genomics (Second Edition) Author: Arthur M. Lesk 2012。
基因组学概论（第二版）[美] A.M.莱斯克 著，薛庆中，胡松年 等 译。
课程评估 ASSESSMENT
19.
评估形式
Type of
Assessment
评估时间
Time
占考试总成绩百分比
% of final
score
违纪处罚
Penalty
备注
Notes
出勤 Attendance
10
课堂表现
Class
Performance
10
小测验
7
Quiz
课程项目 Projects
20
平时作业
Assignments
30
期中考试
Mid-Term Test
期末考试
Final Exam
期末报告
Final
Presentation
30
综述基因组学的应用与发展趋势
Write a review about the application
and development of Genomics
其它（可根据需要
改写以上评估方
式）
Others (The
above may be
modified as
necessary)
20.
记分方式 GRADING SYSTEM
A. 十三级等级制 Letter Grading
B. 二级记分制（通过/不通过） Pass/Fail Grading
课程审批 REVIEW AND APPROVAL
21.
本课程设置已经过以下责任人/委员会审议通过
This Course has been approved by the following person or committee of authority
本课程经生物系本科教学指导委员会审议通过。
This Course has been approved by Undergraduate Teaching Steering Committee of Department of Biology.
