1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请
联系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
蛋白质结构与功能 Protein Structure and Function
2.
授课院系
Originating Department
生物系 Department of Biology
3.
课程编号
Course Code
BIO331
4.
课程学分 Credit Value
3
5.
课程类别
Course Type
专业选修课（生物科学，生物技术，生物信息学专业）
Major Elective Courses (Biological Sciences, Biotechnology, Bioinformatics)
6.
授课学期
Semester
春季 Spring
7.
授课语言
Teaching Language
中英双语 English & Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
魏志毅，生物系， （办公室）88018411
Zhiyi, Wei, Department of Biology, wei.zy@sustech.edu.cn
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
待公布 To be announced
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
15
授课方式
Delivery Method
讲授
Lectures
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
11.
学时数
Credit Hours
30
6
28
64
2
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
BIO201 生物化学 I
Biochemistry I
13.
后续课程、其它学习规划
Courses for which this course
is a pre-requisite
None 无
14.
其它要求修读本课程的学系
Cross-listing Dept.
None 无
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
蛋白质是生命体中最具多样性的大分子，在几乎所有的生命进程中都扮演着必不可少的角色。蛋白质的天然结构是其功能
发挥的基础，不同的结构决定了不同的功能。本课程的目标是通过介绍蛋白质结构与功能之间关系，帮助学生在前期课程
的基础上进一步了解蛋白质如何行使其功能。同时，本课程也强调结构与功能研究的实验基础和技能。通过该课程的学
习，学生能够对蛋白质有更深入的认识，进一步了解生命进程的分子基础，而且能够利用所学到的理论知识和实验技能解
决生物学研究和医学研究中的相关问题。本课程的教学中将采用理论结合实验的方式，有效地促进学生对于课程内容的理
解，并锻炼他们的动手能力，为培养高层次创新人才奠定基础。
Proteins are the most versatile macromolecules in living systems and serve crucial functions in essentially all biological
processes. Our aim is to teach the basic principles governing protein structure-function relationship and the common
techniques in structural and functional studies. With this course, students will have a fundamental understanding of the
structure and function of proteins, an atomic overview of our living system, as well as knowledge and skills to solve
problems in biological and medical research.
16.
预达学习成果 Learning Outcomes
本课程的教学由理论和实验两部分组成，相辅相成，互相促进，提高教学效果。实际教学中包括多项创新型教学方式，包
括：
1、充分结合课程的特点，利用多种先进的 3D 教学模具，帮助学生更好地理解蛋白质结构的特点和与功能之间的关系；
2、应用多种基于电脑的教学游戏和程序，激发学生的学习兴趣和热情；
3、创造性地结合实验来辅助教学，帮助学生了解各种实验手段和技术是如何被用于研究蛋白质结构与功能的关系的；
4、教学内容紧密联系实际，给学生展示如何将学到的知识应用于科学研究和医学中；
5、结合线上和线下的教学模式，通过提高优质的线上教学资料，有效地提高学生的学习深度和广度。
The course teaching consists of theory learning part and experimental part. The teaching combines traditional and
innovative methods, including:
1.Help students to better understand protein structure by using 3D teaching models and practically building 3D structures
by themselves;
2.Apply computer games and programs to stimulate learning interest;
3.Creatively combine the well-designed experiments with theory for students to know why and how different
experimental approaches can be applied in understanding protein structure-function relationship;
4.Tightly link the research and medicine application to the teaching;
5.Effectively combine online and offline teaching by providing related online material for student to expand their leaning
scope.
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
3
Lectures:
Part 1. From Sequence to Structure (8hrs)
具体介绍以下概念（*为重点）
1-1.1 Amino Acids
1-1.2 The Peptide Bond
1-1.3 Interactions that Stabilize Folded Proteins
1-1.4 Properties of Secondary Structures*
Related Experiment: Sequence analysis II
1-2.1 Tertiary Structure
1-2.2 Protein Motifs*
1-2.3 The Protein Domain and Classification*
Related Experiment: Structure DIY, Protein databases
1-3.1 Alpha Domains*
1-3.2 Beta Domains*
1-3.3 Alpha/Beta Domains*
1-3.4 Alpha+Beta and Cross-Linked Domains
1-3.5 Protein Family
Related Experiment: Structure comparison
1-4.1 Protein Folding*
1-4.3 Protein Stability
1-4.3 Quaternary Structure
1-4.4 Protein Assembly
Related Experiment: Surface analysis
Part 2. From Sturcture to Function (8hrs)
具体介绍以下概念（*为重点）
4
2-1.1 Molecular Recognition
2-1.2 Functional Sites
2-1.3 Active Sites
2-1.4 Location and Nature of Binding Sites*
Related Experiment: Identification of functional sites
2-2.1 Catalysis
2-2.2 Proximity and Ground-State Destabilization*
2-2.3 Stabilization of Transition States and Exclusion of Water*
2-2.4 Active-Site Geometry and Chemistry*
2-2.5 Cofactors
2-2.6 Multi-Step Reactions
2-2.7 Multifunctional Enzymes
Related Experiment: Identification of functional sites
2-3.1 Membrane Protein Structure and Folding*
2-3.2 Membrane Protein Prediction
2-3.3 Structure and Function of GPCR
2-3.4 Structure and Function of Ion Channel
2-3.5 Other Membrane Proteins
Related Experiment: Membrane protein analysis
2-4.1 Functional Properties of Structural Proteins
2-4.2 Scaffold Proteins
2-4.3 Flexibility and Protein Function*
Related Experiment: Surface analysis
Part 3. Control of protein function (12hrs)
具体介绍以下概念（*为重点）
5
3-1.1 Protein Interaction Domains
3-1.2 Regulation by Location*
3-1.3 Control by pH and Redox Environment*
Related Experiment: Interaction analysis I
3-2.1 Effector and Allostery *
3-2.2 Competitive Binding and Cooperativity
3-2.3 Conformational Change *
Related Experiment: Structural Comparison
3-3.1 Protein Switches Based on Nucleotide Hydrolysis *
3-3.2 GTPase Switches
3-3.3 ATPase and Motor Protein Switches
3-3.4 Two-Component Signaling System
Related Experiment: Interaction analysis II
3-4.1 Regulation by Post-Translational Modification *
3-4.2 Addition of chemical groups
3-4.3 Methylation and Epigenetics
3-4.4 Glycosylation
3-4.5 Protein Targeting by Lipid Modifications
Related Experiment: Sequence analysis II
3-5.1 Control of Protein Function by Phosphorylation *
3-5.2 Structures of Protein Kinases
3-5.3 Regulation of Signaling Protein Kinases
Related Experiment: Sequence analysis II
3-6.1 Control by Proteolysis *
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3-6.2 Activation of Precursors
3-6.3 Protein Splicing
3-6.4 Ubiquitination and SUMOylation
Related Experiment: Sequence analysis II
Part 4. Structure Determination (2hrs)
4-4.1 Structure Determination Methods
4-4.2 X-Ray Crystallography
4-4.3 NMR
4-4.4 CryoEM
4-4.5 Other Methods
Related Experiment: Structure quality check, Lab tour of instrument for structural studies
教学日历/Schedule
Week
Content
Experiment
1
2
3
4
From sequence to structure
Introduction
Amino acids and secondary structure
Tertiary structure
Protein folding and quaternary structure
Sequence analysis I
Sequence analysis II
5
6
7
8
From structure to function
Functional sites in protein structures
Catalysis
Membrane proteins
Structural proteins
Structure DIY
Protein databases
Structure comparison
Surface analysis
9
10
11
12
13
14
Control of protein function
Environmental controls
Cooperativity and allostery
Regulation switches
Modification regulations
Kinase and signalling regulation
Other regulations
Identification of functional sites
Membrane protein analysis
Interaction analysis I
Interaction analysis II
Protein structure prediction
Structure quality check
15
16
Structure determination
Project presentation
Lab tour of instrument for structural studies
Experimental design
18.
教材及其它参考资料 Textbook and Supplementary Readings
教材/Textbook
Protein Structure and Function, Gregory Petsko & Dagmar Ringe, 2008
参考书/Reference book
Proteins: Structure and Function, David Whitford, 2013
7
课程评估 ASSESSMENT
19.
评估形式
Type of
Assessment
评估时间
Time
占考试总成绩百分比
% of final
score
违纪处罚
Penalty
备注
Notes
出勤 Attendance
课堂表现
Class
Performance
小测验
Quiz
30
13 次实验课+实验考核
课程项目 Projects
20
12 次 Journal Club
平时作业
Assignments
期中考试
Mid-Term Test
期末考试
Final Exam
30
期末报告
Final
Presentation
20
Presentation + Article
其它（可根据需要
改写以上评估方
式）
Others (The
above may be
modified as
necessary)
20.
记分方式 GRADING SYSTEM
A. 十三级等级制 Letter Grading
B. 二级记分制（通过/不通过） Pass/Fail Grading
课程审批 REVIEW AND APPROVAL
21.
本课程设置已经过以下责任人/委员会审议通过
This Course has been approved by the following person or committee of authority
本课程经生物系本科教学指导委员会审议通过。
This Course has been approved by Undergraduate Teaching Steering Committee of Department of Biology.
