1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请
联系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
合成生物学导论与实验基础
Basic Synthetic Biology and Laboratory
2.
授课院系
Originating Department
生物系
Department of Biology
3.
课程编号
Course Code
BIO211
4.
课程学分 Credit Value
2
5.
课程类别
Course Type
专业选修课 （生物科学、生物技术、生物信息学专业）
Major Elective Courses(Biological Sciences, Biotechnology，Bioinformatics Majors)
6.
授课学期
Semester
夏季 Summer
7.
授课语言
Teaching Language
中英双语 English & Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
生物系-黄巍副教授
Department of Biology, Prof. Wei Huang, huangw@sustc.edu.cn
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
无 NA
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
通过全校报名，多轮选拔产生
The students are from preselection
2
授课方式
Delivery Method
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
11.
学时数
Credit Hours
10
28
30（ 集 体 讨 论 ， group
discussion）
74
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
BIO103 生物学原理 Principles of Biology
13.
后续课程、其它学习规划
Courses for which this course
is a pre-requisite
合成生物学导论与实验基础课为生物系学生的专业选修课，同时也是合成生物学元器件、
合成生物学线路及合成生物学系统整合三门通识通修选修课，及参加国际基因机器大赛的
必修课程。
“Basic Synthetic Biology and Laboratory” course is an elective course suitable for
biology majors. It is also a prerequisite for Synthetic Biology Components, Synthetic
Biology Circuits, and Synthetic Biology System Integration Courses, and participation to
iGEM.
14.
其它要求修读本课程的学系
Cross-listing Dept.
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
合成生物学是一种新兴的结合工程手段和科学思想的交叉学科，将对生物问题的理解和生物科学的应用产生巨大的推动作
用。iGEM（国际基因机器大赛)是以本科生为主导的国际合成生物学科研竞赛，有利于培养学生的科研创新能力，合作精
神和多学科交叉学习能力。然而世界知名大学及南科大的 iGEM 比赛往往过分强调比赛，忽略了对本科生合成生物学相关
学科的系统培养。
生物系开设合成生物学导论与实验基础这门课,接受大一及以上的学生，在暑期学期进行强化的合成生物学实验技能培训，
和对合成生物学发展史、经典课题、最新进展、及相关实验及理论方法学进行系统的讲授和研讨，并引导学生自主学习，
互相学习，分组提出、论证合成生物学短期研究课题。
合成生物学导论与实验基础课的目的在于为生物系及相关系学生提供基本的实验室培训、并培养阅读和甄别前沿科研文献
的能力、科学的思维方法和多学科合作学习、研讨的能力。学生将循序渐进地、系统地获得科研实验方法和提出科研问题
的能力，为组队参加国际基因机器大赛打下学科知识和科研合作的基础，也为他们的毕业论文打下良好基础。
Synthetic Biology is a new scientific discipline that integrates engineering approach and scientific thinking. It will provide
driving force for understand basic biological questions as well as application of biological sciences. International Genetic
Engineered Machine (iGEM) is a prominent international synthetic biology research competition primarily run by
undergraduate students. It promotes innovation, cooperativity and multidisciplinary trainings to undergraduate students.
However the top universities in the world, SUSTC included, are too focused on the competition itself and neglect the
systematic undergraduate educations in synthetic biology and related disciplinary.
The Department of Biology offers Basic Synthetic Biology and Laboratory Course every summer to provide students from
Biology and other departments with basic laboratory training, and training in critical studying scientific literatures, and
scientific and logical reasoning, and capability to team learning and multidisciplinary exposures.
The students are expected to gradually learning the scientific experimental research methodology, and the capability to
raise sound scientific questions. It will also lead to assemble the SUSTC iGEM team. This course will also lay the
3
foundation for their own final year project research.
16.
预达学习成果 Learning Outcomes
本课程完成后，学生将能够：
熟悉并了解生物实验室。
熟悉并了解分子生物学与合成生物学实验基础。
通过授课、引导性教学与研讨，理论指导和实验，探索所选择的专业。
明确并评估自己将来的学术和职业目标。
On completion of this course, students are expected to:
Have a basic, practical understanding of the biology research laboratory.
Have an understanding of the general techniques employed carried out in biology laboratory biological research.
Gain practical work experience by applying classroom-learned concepts on the job in assigned lab research work.
Explore the chosen major.
Clarify and evaluate academic and career goals.
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
Lectures
1-2hr. Engineering principle of Synthetic Biology, The techniques of gene editing
3-4hr. Synthetic biology based on known engingeering principle, Synthetic Biology to test unknown principle, The
applications of Synthetic Biology
5-6hr. Introduction of iGEM 和 Biobricks
7-16hr. Seminars on classic synthetic biology papers
17-26hr，Students presentation of previous iGEM projects won major awards
27-40hr，Student discuss assigned and self-picked most recent synthetic biology papers
41-46hr， Brainstorming， students forming their own synthetic biology project, formulize the research plan, including
experimental and theoretical parts.
Laboratory
Day1（4hrs）
4
lecture on making competent cells，
Inoculate DH5 to LB broth (without antibiotics) and incubate at 18°C for ~3 days,
Inoculate bacteria with plasmids to LB broth and incubate at 37°C for 16 h
Day2（8hrs）
Lecture on Plasmid purification and enzyme digestion
Plasmid purification，
make enzyme digestion system and incubate at 37°C for ~3 h
lecture on preparing medium & pH meter
prepare TB (Inoue) - pH meter, filter
prepare TAE buffer
prepare antibiotics stock solution - filter
store the enzyme digestion system at 4°C
Day 3 （8hrs）
Lecture on electrophoresis and gel extraction
*make agarose gel
*Prepare LB broth medium & LB agar plate - autoclave
*electrophoresis
*gel extraction with kit and electrophoresis
Lecture on ligation and transformation
*pour LB agar plate (antibiotics)
*ligation and transformation
Day 4 (8hrs)
Lecture on screening PCR
*make PCR system
*make agarose gel
Lecture on spectrophotometer
*measure OD600
*electrophoresis
5
*measure OD600
*prepare competent cells
18.
教材及其它参考资料 Textbook and Supplementary Readings
合成生物学导论 Introduction to Synthetic Biology,
宋凯编著，黄熙泰主审，高等教育出版社，2010， ISBN 978-7-03-026731-3
以及相关合成生物学前沿文献
课程评估 ASSESSMENT
19.
评估形式
Type of
Assessment
评估时间
Time
占考试总成绩百分比
% of final
score
违纪处罚
Penalty
备注
Notes
出勤 Attendance
课堂表现
Class
Performance
60 The evaluation
provided by the
supervising professor,
on the basis of the
following aspects:
Performance in
Laboratory work;
Attendance and
participation in
lecture , performance
in presentations .
由教授来评估：实验
室的工作表现；出勤情
况；在 pre 中的表现
小测验
Quiz
课程项目 Projects
平时作业
Assignments
期中考试
Mid-Term Test
期末考试
Final Exam
期末报告
Final
Presentation
40 The final PPT-
based oral
presentation given by
the student on their
research proposal on
synthetic biology.
此部分评估学生的口
头报告（关于课程生物
学研究）
其它（可根据需要
改写以上评估方
式）
Others (The
above may be
6
modified as
necessary)
20.
记分方式 GRADING SYSTEM
A. 十三级等级制 Letter Grading
B. 二级记分制（通过/不通过） Pass/Fail Grading
课程审批 REVIEW AND APPROVAL
21.
本课程设置已经过以下责任人/委员会审议通过
This Course has been approved by the following person or committee of authority
本课程经生物系本科教学指导委员会审议通过。
This Course has been approved by Undergraduate Teaching Steering Committee of Department of Biology.