1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请
联系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
细胞生物学 Cell Biology
2.
授课院系
Originating Department
生物系
Department of Biology
3.
课程编号
Course Code
BIO206-15
4.
课程学分 Credit Value
4
5.
课程类别
Course Type
专业核心课(生物科学、生物技术、生物信息学）
Major Core Courses (Biological Sciences, Biotechnology, Bioinformatics)
6.
授课学期
Semester
春季 Spring/ 秋季 Fall
7.
授课语言
Teaching Language
英文 English / 中英双语 English & Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
张严冬，助理教授，生物系
zhangyd@sustc.edu.cn
ZHANG, Yandong , Assistant Professor, Department of Biology
Peter Pimpl, 副教授，生物系
pimpl@sustc.edu.cn
Peter Pimpl， Associate Professor, Department of Biology
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
待公布 To be announced
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
授课方式
Delivery Method
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
11.
学时数
Credit Hours
8 Review and Mid-term
Exam
64
2
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
BIO102A 普通生物学 General Biology
13.
后续课程、其它学习规划
Courses for which this course
is a pre-requisite
本课程 为生物系专 业核心课，是 BIO208 细胞生 物学实验、 BIO302 现代生物技术 、
BIO304 系统生物学的先选课程；高级细胞生物学、干细胞与再生生物学、分子药理学、
免疫学的先修课程；其它非生物学专业学生如果想学习有关细胞的功能调控，也可选修本
课程。
This course is a core course in biology, a prerequisite course in Cell Biology Laboratory,
Modern Biotechnology, and Systems Biology; a pre-requisite course of Advanced Cell
Biology, Stem Cell and Regenerative Biology, Molecular Pharmacology, Immunology; It
should however also be suitable for non-specialists, i.e. for all those medical students to
gain a certain amount of familiarity.
14.
其它要求修读本课程的学系
Cross-listing Dept.
无 None
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
细胞生物学是生物化学，遗传学及分子生物学在细胞水平的整合，目的是： 1. 了解细胞中几种重要大分子包括 DNA，
RNA，蛋白质，脂类及它们所构成的功能复合物的结构，及其在生命活动中的重要功能； 2. 细胞中各种亚细胞器的结构功
能， 认识它们在执行各项生命活动中的协调关系；3.掌握细胞的各项有序生命活动，如物质运输，细胞周期，细胞通讯的
基本原理； 4.了解细胞死亡，癌变的成因, 治疗机制； 5. 细胞骨架结构功能，细胞外基质与细胞之间的相互联系，细胞如
何整合为组织；6.了解干细胞及神经细胞等细胞生物学一些前沿领域。 结合研究实例，掌握细胞生物学各个研究领域的重
要发现。 同时通过讲解目前一些常用的细胞生物学研究方法， 让学生理解细胞生物学的研究内容、研究方法和研究思路。
Cell Biology is the integration of several basic subjects such as biochemistry, genetics and molecular biology on the level
of cell. The aim is to: 1. Understand the basic structures and functions for biomolecules as well as their complexes
including RNA/DNA, protein, lipid in cells. 2. Learn various cellular organelles and their structures as well as functions. 3.
Learn the basic concepts in molecular transport, cell communication, cell cycle and cell growth. 4. Understand principles
for cell death, cancer development as well as cancer treatment. 5. Learn the structure and function for cytoskeleton,
understand how cells integrate into tissues and the composition and function of extracellular matrix. 6. Know some basic
concepts for stem cell research and neurobiology. Finally, through the introduction of common experimental methods and
classical experiments in Cell Biology, let the students understand the basic research methodology and rationales behind
research designing.
16.
预达学习成果 Learning Outcomes
学生会对细胞有一个系统的了解。 这主要包括：细胞的结构和功能；细胞生物学的研究方法；膜生物化学及小分子运输；
蛋白转运；细胞信号转导；细胞骨架；细胞黏附及胞外基质；细胞周期；细胞死亡和癌症生物学等。
Students will have a systematic understanding for cell, covering the following aspects: cell structure and function,
methodology to study cell biology, membrane biochemistry and transport, protein sorting and trafficking, cell-cell
communication/signalling, cytoskeleton, cell adhesion, cell cycle, cell death, cancer biology.
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
3
Week/Lecture
No.
讲座题目
Lecture Topic
课本阅
读
Chapter
reading
学时
Hours
具体讲座内容
Lecture content
Week 1/ 1
细胞的概论
Introduction to the
cell
Ch.1 &2
2 学时
2 hours
细胞生物学发展简史，细胞理论的提出
细胞的共性，细胞多样性中基因进化原理
原核细胞和真核细胞的对比
细胞生物学中的常用模式生物及其应用
A short history of cell biology and cell
theory
The common features of all cells and the
variation of cell genomes
Compare and contrast prokaryotic and
eukaryotic cells
Model systems in cell biology studies and
their applications
Week 1/ 2-
Week 2/3
细胞生物学研究方法
Methods to study
Cell Biology
Ch.9
4 学时
4 hours
显微镜的原理和应用（普通光学显微镜，相差显微
镜，DIC 显微镜，荧光显微镜，激光共聚焦显微镜，
电子显微镜，超分辨显微镜和冷冻电镜）
Visualizing cells ( bright field, dark field,
phase contrast, differential interference,
fluorescence, confocal microscopy, electron
microscopy, nanoscopy and cyro-EM microscopy
techniques and their applications)
细胞的基本组成成分和分离分析方法（蛋白质，DNA
和 RNA 的分析及分离技术）
The major components of cells (DNA, RNA and
proteins). The isolation of proteins and
common methods to analyze proteins
(chromatography, centrifugation and SDS-PAGE,
2-D gel for proteome analysis). The isolation
of DNA and RNA from cells, the application of
Agarose gel electrophoresis, DNA cloning, PCR
and RT-PCR, the use of DIG- and Biotin for
nucleic acid labelling)
细胞培养和分离的常用方法　(从组织中分离细胞的
常用方法，细胞的常用购买渠道和常用细胞的培养基
成分，二维和三维细胞的培养体系原理和优缺点)
How to obtain pure cell culture from tissues
4
The basic components in cell medium
Compare and contrast 2-D and 3-D cell culture　
systems.
Week 2/4
细胞膜结构 I
Membrane Structure
I
Ch.10
2 学时
2 hours
生物膜的概论
生物膜的基本结构和功能
生物膜脂类分子的研究方法
生物膜中的脂双层中的主要构成成分，其结构和功能
生物膜的组装
Overview of membrane
Common features of membrane and its functions
Methods to study lipid molecules
Major components of Lipid bilayer as well as
their structures and functions.
Membrane assembly
Week 3/5
细胞膜结构 II
Membrane Structure
II
Ch.10
2 学时
2 hours
生物膜中的蛋白质种类，结构，功能和分析方法, 去
污剂的性质，种类和其在膜生物学中的应用
Categories of membrane proteins
Methods to study membrane proteins
The features of membrane proteins, their
structures and functions
Hydropathy plots to predict membrane protein
structures.
The use of detergents to study membrane
proteins, micelle formation
Week 3/6
小分子运输 I
Membrane transport
I
Ch.11
2 学时
2 hours
膜的通透性和内外小分子浓度的差别
简单渗透，主动运输和被动运输
协助运输的两种蛋白：channel and transporter
小分子物质运输的原理，水分子，离子通道（水通道
和钾离子通道）　
Selective transportation of small molecules
Simple diffusion , Active transport and
passive transport