课程详述

COURSE SPECIFICATION

以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请

联系授课教师。

The course information as follows may be subject to change, either during the session because of unforeseen

circumstances, or following review of the course at the end of the session. Queries about the course should be

directed to the course instructor.

课程名称 Course Title

细胞生物学 Cell Biology

授课院系

Originating Department

生物系

Department of Biology

课程编号

Course Code

BIO206-15

课程学分 Credit Value

课程类别

Course Type

专业核心课(生物科学、生物技术、生物信息学）

Major Core Courses (Biological Sciences, Biotechnology, Bioinformatics)

授课学期

Semester

春季 Spring/ 秋季 Fall

授课语言

Teaching Language

英文 English / 中英双语 English & Chinese

授课教师、所属学系、联系方

式（如属团队授课，请列明其

他授课教师）

Instructor(s), Affiliation&

Contact

（For team teaching, please list

all instructors）

张严冬，助理教授，生物系

zhangyd@sustc.edu.cn

ZHANG, Yandong , Assistant Professor, Department of Biology

Peter Pimpl, 副教授，生物系

pimpl@sustc.edu.cn

Peter Pimpl， Associate Professor, Department of Biology

实验员/助教、所属学系、联系

方式

Tutor/TA(s), Contact

待公布 To be announced

10.

选课人数限额(可不填)

Maximum Enrolment

（Optional）

授课方式

Delivery Method

讲授

Lectures

习题/辅导/讨论

Tutorials

实验/实习

Lab/Practical

其它(请具体注明)

Other（Please specify）

总学时

Total

11.

学时数

Credit Hours

8 Review and Mid-term

Exam

12.

先修课程、其它学习要求

Pre-requisites or Other

Academic Requirements

BIO102A 普通生物学 General Biology

13.

后续课程、其它学习规划

Courses for which this course

is a pre-requisite

本课程为生物系专业核心课，是 BIO208 细胞生物学实验、 BIO302 现代生物技术、

BIO304 系统生物学的先选课程；高级细胞生物学、干细胞与再生生物学、分子药理学、

免疫学的先修课程；其它非生物学专业学生如果想学习有关细胞的功能调控，也可选修本

课程。

This course is a core course in biology, a prerequisite course in Cell Biology Laboratory,

Modern Biotechnology, and Systems Biology; a pre-requisite course of Advanced Cell

Biology, Stem Cell and Regenerative Biology, Molecular Pharmacology, Immunology; It

should however also be suitable for non-specialists, i.e. for all those medical students to

gain a certain amount of familiarity.

14.

其它要求修读本课程的学系

Cross-listing Dept.

无 None

教学大纲及教学日历 SYLLABUS

15.

教学目标 Course Objectives

细胞生物学是生物化学，遗传学及分子生物学在细胞水平的整合，目的是： 1. 了解细胞中几种重要大分子包括 DNA，

RNA，蛋白质，脂类及它们所构成的功能复合物的结构，及其在生命活动中的重要功能； 2. 细胞中各种亚细胞器的结构功

能，认识它们在执行各项生命活动中的协调关系；3.掌握细胞的各项有序生命活动，如物质运输，细胞周期，细胞通讯的

基本原理； 4.了解细胞死亡，癌变的成因, 治疗机制； 5. 细胞骨架结构功能，细胞外基质与细胞之间的相互联系，细胞如

何整合为组织；6.了解干细胞及神经细胞等细胞生物学一些前沿领域。结合研究实例，掌握细胞生物学各个研究领域的重

要发现。同时通过讲解目前一些常用的细胞生物学研究方法，让学生理解细胞生物学的研究内容、研究方法和研究思路。

Cell Biology is the integration of several basic subjects such as biochemistry, genetics and molecular biology on the level

of cell. The aim is to: 1. Understand the basic structures and functions for biomolecules as well as their complexes

including RNA/DNA, protein, lipid in cells. 2. Learn various cellular organelles and their structures as well as functions. 3.

Learn the basic concepts in molecular transport, cell communication, cell cycle and cell growth. 4. Understand principles

for cell death, cancer development as well as cancer treatment. 5. Learn the structure and function for cytoskeleton,

understand how cells integrate into tissues and the composition and function of extracellular matrix. 6. Know some basic

concepts for stem cell research and neurobiology. Finally, through the introduction of common experimental methods and

classical experiments in Cell Biology, let the students understand the basic research methodology and rationales behind

research designing.

16.

预达学习成果 Learning Outcomes

学生会对细胞有一个系统的了解。这主要包括：细胞的结构和功能；细胞生物学的研究方法；膜生物化学及小分子运输；

蛋白转运；细胞信号转导；细胞骨架；细胞黏附及胞外基质；细胞周期；细胞死亡和癌症生物学等。

Students will have a systematic understanding for cell, covering the following aspects: cell structure and function,

methodology to study cell biology, membrane biochemistry and transport, protein sorting and trafficking, cell-cell

communication/signalling, cytoskeleton, cell adhesion, cell cycle, cell death, cancer biology.

17.

课程内容及教学日历（如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明

主讲人）

Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if

this is a team teaching or module course.)

Week/Lecture

No.

讲座题目

Lecture Topic

课本阅

读

Chapter

reading

学时

Hours

具体讲座内容

Lecture content

Week 1/ 1

细胞的概论

Introduction to the

cell

Ch.1 &2

2 学时

2 hours

细胞生物学发展简史，细胞理论的提出

细胞的共性，细胞多样性中基因进化原理

原核细胞和真核细胞的对比

细胞生物学中的常用模式生物及其应用

A short history of cell biology and cell

theory

The common features of all cells and the

variation of cell genomes

Compare and contrast prokaryotic and

eukaryotic cells

Model systems in cell biology studies and

their applications

Week 1/ 2-

Week 2/3

细胞生物学研究方法

Methods to study

Cell Biology

Ch.9

4 学时

4 hours

显微镜的原理和应用（普通光学显微镜，相差显微

镜，DIC 显微镜，荧光显微镜，激光共聚焦显微镜，

电子显微镜，超分辨显微镜和冷冻电镜）

Visualizing cells ( bright field, dark field,

phase contrast, differential interference,

fluorescence, confocal microscopy, electron

microscopy, nanoscopy and cyro-EM microscopy

techniques and their applications)

细胞的基本组成成分和分离分析方法（蛋白质，DNA

和 RNA 的分析及分离技术）

The major components of cells (DNA, RNA and

proteins). The isolation of proteins and

common methods to analyze proteins

(chromatography, centrifugation and SDS-PAGE,

2-D gel for proteome analysis). The isolation

of DNA and RNA from cells, the application of

Agarose gel electrophoresis, DNA cloning, PCR

and RT-PCR, the use of DIG- and Biotin for

nucleic acid labelling)

细胞培养和分离的常用方法　(从组织中分离细胞的

常用方法，细胞的常用购买渠道和常用细胞的培养基

成分，二维和三维细胞的培养体系原理和优缺点)

How to obtain pure cell culture from tissues

The basic components in cell medium

Compare and contrast 2-D and 3-D cell culture　

systems.

Week 2/4

细胞膜结构 I

Membrane Structure

Ch.10

2 学时

2 hours

生物膜的概论

生物膜的基本结构和功能

生物膜脂类分子的研究方法

生物膜中的脂双层中的主要构成成分，其结构和功能

生物膜的组装

Overview of membrane

Common features of membrane and its functions

Methods to study lipid molecules

Major components of Lipid bilayer as well as

their structures and functions.

Membrane assembly

Week 3/5

细胞膜结构 II

Membrane Structure

Ch.10

2 学时

2 hours

生物膜中的蛋白质种类，结构，功能和分析方法, 去

污剂的性质，种类和其在膜生物学中的应用

Categories of membrane proteins

Methods to study membrane proteins

The features of membrane proteins, their

structures and functions

Hydropathy plots to predict membrane protein

structures.

The use of detergents to study membrane

proteins, micelle formation

Week 3/6

小分子运输 I

Membrane transport

Ch.11

2 学时

2 hours

膜的通透性和内外小分子浓度的差别

简单渗透，主动运输和被动运输

协助运输的两种蛋白：channel and transporter

小分子物质运输的原理，水分子，离子通道（水通道

和钾离子通道）　

Selective transportation of small molecules

Simple diffusion , Active transport and

passive transport

channels and transporters

离子通道　 ( aqueous channel, Potassium

channel )

Week 4/7 Quiz

1 Homework 1

小分子运输 II

Membrane transport

Ch.11

2 学时

2 hours

小分子运输通道 transporters (uniporter,

symporter, anti-porter)

ATP 泵（钙离子泵，钠钾泵）

ABC transporter,

膜电势的产生原理（membrane potential）

神经冲动的传导（action potential and electron

impulse transmission）

离子通道的干扰药物，麻醉剂，神经干扰药物等。

（drugs inhibiting ion channels）

Week 4/8

蛋白质转运 I

protein sorting I

Ch.12

2 学时

2 hours

细胞区室（cell compartment）

核质物质运输（nuclear and cytoplasmic

transportation）

线粒体和叶绿体的蛋白转运（protein

transportation to mitochondria and

chloroplast）

过氧化物酶体的蛋白转运（transportation to

peroxisomes ）

内质网的物质转运（transportation to

endoplasmic reticulum）

脂分子的合成和运输（bio-synthesis of lipid

molecules and transportation）

Week 5/9

蛋白质转运 II

Protein sorting II

Ch.13

2 学时

2 hours

囊泡运输概论（overview of vesicle

trafficking ）

囊泡运输的研究方法（methods to study vesicle

trafficking

从内质网到高尔基体的蛋白转运（transportation

from ER to Golgi apparatus）

从高尔基体到内质网的蛋白转运（transportation

from Golgi apparatus to ER）

内吞（endocytosis ）

胞吐（exocytosis ）

Week 5/10

线粒体和叶绿体

Ch.14

2 学时

线粒体的结构，区室和功能

Mitochondria and

Chloroplast

2 hours

叶绿体的结构，区室和功能

线粒体和叶绿体的遗传系统

化学渗透欧联－生物能的产生

Mitochondrion structure and its compartment

Plastid and chloroplast structure/ function

Genetic systems in mitochondria and

chloroplasts

Energy conversion---chemiosmosis coupling

Week 6/11

蛋白质功能调控

Protein functional

regulation

Ch.14

2 学时

2 hours

蛋白质的复杂性

蛋白活性的调控

蛋白稳态的调控

蛋白的泛素化和蛋白讲解

蛋白的错误折叠和人类疾病

Protein complexity

Control of protein activity

Control of protein steady-state level

Ubiquitination and protein degradation in the

proteasome

Protein misfolding and human diseases

Week 6/12

Quiz 2

Homework 2

细胞通讯 I

Cell

communication/signa

ling I

Ch.15

2 学时

2 hours

细胞通讯概论

细胞内的细胞转导

细胞表面受体介导的通讯

细胞信号转导的一般研究方法

细胞通讯动力学及正反馈和负反馈调控

Overview of cell signaling

Intracellular signaling

General principles of cell surface signaling

Several methods to study cell signaling

Positive and negative feedback in signaling

and signaling kinetics

Week 7/13

细胞通讯 II

Cell

communication/signa

ling II

Ch.15

2 学时

2 hours

Ｇ蛋白耦联受体的发现和简史

Ｇ蛋白耦联受体的概论

Ｇ蛋白偶联受体的分离及代表途径的详解

Discovery of G-protein coupled receptor and

short history

Overview of GPCR signaling

Category of GPCRs and typical examples with

detailed introduction

Desensitization of GPCR signaling

Week 7/14

细胞通讯 III

Cell

communication/signa

ling III

Ch.15

2 学时

2 hours

酶连受体信号通路概论

酪氨酸激酶受体通路

酪氨酸相关激酶受体通路

丝氨酸、苏氨酸激酶受体通路

组氨酸激酶相关受体通路

Overview of enzyme –linked receptor

signalling:

receptor tyrosine kinase (RTK)

Tryosine-kinase-associated receptors

receptor Ser/Thr kinase

Histidine-kinase-associated receptors

Week 8/15

复习 I

Review session I

2 学时

2 hours

Week 8/16

期中考试

Midterm Exam

2 学时

2 hours

Week 9/17

细胞骨架 I

Cytockeleton I

Ch.16

2 学时

2 hours

细胞骨架总论

微丝和肌动蛋白结构

肌动蛋白微丝的动态特征

微丝组装的原理

Overview of cytoskeleton

Microfilament and actin structures

Dynamics of actin filaments

Mechanisms of actin filament assembly

Organization of actin-based cellular structure

Week 9/18

细胞骨架 II

Cytoskeleton II

Ch.16

2 学时

2 hours

肌球蛋白的分类，结构和功能

肌球蛋白在微丝的移动原理

细胞的移动原理，信号通路和细胞的趋化运动

Myosins: actin-based motor proteins

Mysoin-powered movements

Cell migration: mechanisms, signaling, and

chemotaxis

Week 10/19

细胞骨架 III

Cytoskeleton III

Ch.16

2 学时

2 hours

微管的结构和组装

微管组装的动态原理

微管组装的调控原理

微管的两种马达蛋白结构和功能

纤毛和鞭毛的结构及运动原理

中间纤维

Microtubule structure and organization

Microtubule dynamics

Regulation of microtubule assembly

Kinesins and Dyneins: microtubule-based motor

proteins

Cilia and flagella

Intermediate filaments

Week 10/20

细胞周期 I

Cell cycle I

Ch.17

2 学时

2 hours

细胞周期概论

细胞周期的研究方法

细胞周期的调控系统

细胞周期中的 S 期

Overview of the cell cycle

Common methods to study cell cycle

Introduction to the cell cycle control system

S phase

Week 11/21

Quiz 3

Homework 4

细胞周期 II

Cell cycle II

Ch.17

2 学时

2 hours

细胞周期中的有丝分裂期

细胞分裂

细胞生长和细胞分裂的协调和失调

Mitosis

Cytokinesis

Control of cell division and cell growth

Week 11/22

细胞凋亡

Apoptosis

Ch.18

2 学时

2 hours

细胞死亡的种类和概述

细胞凋亡的特征和分析方法

细胞凋亡的发现和研究简史

细胞凋亡的机理（外因凋亡和内因凋亡，凋亡的重

要因子 CASPASE 及调控基因 Bcl-2 基因家族）

细胞凋亡的失调和人类疾病的发生

Overview of programmed cell death

Methods in apoptosis identification

Brief history of apoptosis

Mechanisms （Caspases, Bcl-2 family genes）

Dysregulation of apoptosis and human diseases

Week 12/23

细胞自噬

Autophagy

Ch.18

2 学时

2 hours

细胞自噬的秘密

细胞自噬系统的发现简史

细胞自噬的基本原理

细胞自噬的失调和人类疾病的关联

The mystery of autophagy

Discovery of the autophagy machinery

Basic components in autophagy and functions

Autophagy in health and diseases

Week 12/24

Quiz 4

Homework4

复习 II

Review Session II

2 学时

2 hours

Week 13/25

细胞连接,细胞黏附和

胞外基质 I

Cell Junctions,

Cell adhesion, and

ECM I

Ch.19

2 学时

2 hours

细胞整合为组织的概述

细胞之间连接的种类

钙黏连蛋白和细胞与细胞连接

整合素及细胞与基质连接

Overview of cell-cell and cell-ECM junction

and adhesion

Cadherins and cell- cell adhesion

Integrins in cell-ECM adhesion

Week 13/26

细胞连接,细胞黏附和

胞外基质 II

Cell Junctions,

Cell adhesion, and

ECM II

Ch.19

2 学时

2 hours

细胞致密连接的主要蛋白种类，结构和功能

细胞孔道连接的主要蛋白种类，结构和功能

细胞基膜的组成，功能和分布

Occluding junctions

Channel-forming Junctions

Basal Lamina

Week 14/27

细胞连接,细胞黏附和

胞外基质 III

Ch.19

2 学时

胞外基质的概论

胞外基质的组成成分及介绍（蛋白聚糖，糖蛋白，胶

Cell Junctions,

Cell adhesion and

ECM III

2 hours

原蛋白，弹性蛋白，黏连蛋白）

胞外基质的生物合成和讲解

Overview of ECM

The making and composition of ECM

GAGs

Hyaluronan

proteoglycans

collagens

elastin

fibronectin

The degradation of ECM

Week 14/28

Quiz 5

Homework 5

癌症 I

Cancer I

Ch.20

2 学时

2 hours

癌症的概论

癌症的特征

癌症产生的诱因

The nature of Cancer

Properties of cancer

Cause of cancer

Week 15/29

癌症 II

Cancer II

Ch.20

2 学时

2 hours

癌症重要基因

癌症发生的多步骤机理

癌症干细胞

癌症转移

癌症的传统治疗和最新治疗方法

Cancer critical genes

Multi-step tumorigenesis

Cancer stem cells

Cancer metastasis

Cancer treatment

Week 15/30

干细胞

Stem cell

Handout

2 学时

2 hours

干细胞的特征的分类介绍

干细胞在多细胞生物发育中的功能

细胞的不对称分裂

干细胞在多细胞生物中的分布及微环境对干性的维持

Stem cell introduction

Early metazoan development and embryonic stem

cells

Asymmetric cell division

Stem cells and niches in multicellular

organisms

Week 16/31

神经细胞

Neurobiology

Handout

2 学时

2 hours

神经细胞的分类：神经元和胶质细胞

神经元的电信号通路

胶质细胞的分类功能和分布

血脑屏障

神经髓鞘的结构功能

神经突触

Categories of nerve cells: neuron and glia

Circuit of neuron impulse ( motor neuron,

interneuron, sensory neuron, etc)

Category of glia cells , their structures,

functions.

Blood-brain barrier

Neuron sheath structure and function

Synapse

Week 16/32

复习 Review session

III

2 学时

2 hours

Final weeks

Final Exam

18.

教材及其它参考资料 Textbook and Supplementary Readings

指定教材: Molecular Biology of the Cell, 5th edition, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith

Roberts and Peter Walter. Garland Science

参考教材：Molecular Cell Biology, 7th edition, Harvey Lodish, Chris Kaiser, Anthony Bretscher, Angelicka Amon,

Arnold BERK, Monty Krieger, Hidde Ploeph, Matthew Scott. Macmillan

课程评估 ASSESSMENT

19.

评估形式

Type of

Assessment

评估时间

Time

占考试总成绩百分比

% of final

score

违纪处罚

Penalty

备注

Notes

出勤 Attendance

2.5

课堂表现

Class

Performance

2.5

小测验

Quiz

课程项目 Projects

平时作业

Assignments

期中考试

Mid-Term Test

期末考试

Final Exam

期末报告

Final

Presentation

其它（可根据需要

改写以上评估方

式）

Others (The

above may be

modified as

necessary)

20.

记分方式 GRADING SYSTEM

 A. 十三级等级制 Letter Grading

 B. 二级记分制（通过/不通过） Pass/Fail Grading

课程审批 REVIEW AND APPROVAL

21.

本课程设置已经过以下责任人/委员会审议通过

This Course has been approved by the following person or committee of authority

本课程经生物系本科教学指导委员会审议通过。

This Course has been approved by Undergraduate Teaching Steering Committee of Department of Biology.