1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请
联系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
微生物实验 Microbiology Laboratory
2.
授课院系
Originating Department
生物系 Biology Department
3.
课程编号
Course Code
BI0205
4.
课程学分 Credit Value
2
5.
课程类别
Course Type
专业选修课（生物科学、生物技术、生物信息学专业）
Major Elective Courses（Biological Sciences, Biotechnology, Bioinformatics Majors）
6.
授课学期
Semester
秋季 Fall
7.
授课语言
Teaching Language
中英双语 English & Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
all instructors）
吕沫，生物系，lv.m3@sustc.edu.cn
LVMo, Biology Department, lv.m3@sustc.edu.cn
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
张敏，生物系，zhangm6@sustc.edu.cn
Zhang Min, Biology Department, zhangm6@sustc.edu.cn
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
2
授课方式
Delivery Method
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
11.
学时数
Credit Hours
64
64
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
要求先修 BIO104 普通生物学实验；学习本实验课的同时学习 BIO 203 微生物学或者
ESE301 环境微生物学。
Pre-study General Biology Laboratory, and study the theory courses Microbiology or
Environmental Microbiology with the experiments course in the same time.
13.
后续课程、其它学习规划
Courses for which this course
is a pre-requisite
14.
其它要求修读本课程的学系
Cross-listing Dept.
环境科学工程 Environmental Science Engineering
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
微生物学是一门实践和应用性很强的学科，而微生物学实验是微生物学课程中重要的教学环节。本课程以动手实验为主，
讲授为辅，通过实验课教学巩固和补充课堂上讲授的理论知识，使学生掌握基本的实验操作技术和科学实验的概念，比如
学习显微镜的使用，无菌操作技术和纯培养等。微生物实验课是非常有价值的课程，它会让学生近距离的学习和研究微生
物。而且，给学生提供机会去学习特殊的实验技巧，鉴别微生物的物种。并且学生可以掌握对观察实验现象，统计实验数
据和分析实验数据的能力。并将所学的微生物理论知识有机的结合起来，为微生物的实际应用打下坚实基础。
Microbiology is a highly practical and applied disciplines, and microbiology laboratory is an important teaching curriculum.
This course gives you a chance to learn the special techniques and is supplemented with lectures. It will help students to
understand and add some knowledge of the theory course. The goal is to teach students basic techniques and concepts:
the use of the microscope and the aseptic and pure culture techniques. A microbiology laboratory is valuable because it
actually gives you a chance to see and study microorganisms firsthand. In addition, it provides you with the opportunity to
learn the special techniques used to study and identify these organisms. The ability to make observations, record data,
and analyze results is useful throughout life. These exercises are also designed to help students understand basic
biological concepts that are the foundation for applications in all life science fields.
16.
预达学习成果 Learning Outcomes
该实验课让学生掌握微生物学中常用新的实验技能，受到观察某些微生物学现象的训练，同时培养学生从事科学研究工作
的良好作风和工作习惯，逐步培养学生将学到的微生物规律和知识应用到各个领域。微生物实验课为学生打开了微生物的
大门，希望学生能够掌握这门知识，并启发学生对这个领域的探索热情
The laboratory is chosen to give students an opportunity to learn new techniques and to expose them to a variety of
experiences and observations. At the same time, it can train the students a good style and work habits in scientific
research work. The principles and techniques demonstrated in microbiology laboratory can be applied to many fields.
This course is an introduction to the microbial world, and we hope students will find it useful and interesting.
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
3
第一章微生物学实验安全介绍和课程简介 学时：4 学时
Lab 1 Introduction to microbiology Lab Safety and Lab course Hours: 4 h
主要内容：
Outline:
1.介绍微生物实验室规章制度，微生物实验操作安全。
Introduce the rules and regulations of microbiology laboratory and the safety of microbiology experiment operation.
2.介绍微生物实验课程内容和考核方式。
Introduce the contents and assessment methods of Microbiology laboratory course.
教学要求：
Requirements:
[掌握]
1.微生物实验课开始实验之前的注意事项
Notes before starting the experiment in Microbiology laboratory course
2.微生物实验室的安全配套设施的使用方法
How to use the safety supporting facilities in Microbiology laboratory
[熟悉]
1.安全事故的应急处理方法
Emergency handling methods for safety accidents
2.课程考核方式
Course assessment method
3.Lab report 和 Notebook 的书写要求
Written request of Lab report and Notebook
[了解]
1.课程内容
course content
重点、难点：微生物实验课的注意事项。Lab report 和 Notebook 的书写要求。
Key and difficulty: Notes before starting the experiment in Microbiology laboratory course. Written request of Lab report
and Notebook
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第二章微生物无菌操作技术和培养基的制备 学时：5 学时
Lab 2 Aseptic Technique and Microbiological Culture Media Preparation Hours: 5 h
主要内容：
Outline:
1.微生物实验无菌操作技术简介。
Learn the common aseptic techniques.
2.培养基分类和功能。
Medium classification and function
3.学习培养基配制的方法和步骤。
Learn the method and procedure of medium preparation.
教学要求：
Requirements:
[掌握]
1.灭菌和消毒
Sterilization and disinfection
2.准备不同类型的培养基，培养基的高压灭菌方法。
Prepare different types of media, and Autoclave method of culture media.
[熟悉]
1.学习普通培养基、富集培养基、选择性培养基和筛选性培养基的功能。
Learn the function of general, enriched, selective, and differential media.
[了解]
1.培养基的基本组成元素和用途。
Basic elements and functions of the medium.
重点、难点：准备不同类型的培养基，培养基的高压灭菌方法。
Key and difficulty: Prepare different types of media, and Autoclave method of culture media.