1
课程详述
COURSE SPECIFICATION
以下课程信息可能根据实际授课需要或在课程检讨之后产生变动。如对课程有任何疑问，请
联系授课教师。
The course information as follows may be subject to change, either during the session because of unforeseen
circumstances, or following review of the course at the end of the session. Queries about the course should be
directed to the course instructor.
1.
课程名称 Course Title
生物实验室安全与个人防护 Biological Lab Safety and Personal Protection
2.
授课院系
Originating Department
生物系 Department of Biology
3.
课程编号
Course Code
BIO112
4.
课程学分 Credit Value
1
5.
课程类别
Course Type
专业选修课 Major Elective Courses
6.
授课学期
Semester
春季 Spring / 秋季 Fall
7.
授课语言
Teaching Language
中英双语 English & Chinese
8.
授课教师、所属学系、联系方
式（如属团队授课，请列明其
他授课教师）
Instructor(s), Affiliation&
Contact
（For team teaching, please list
allinstructors）
余春红，工程师，生物系
第一教学楼 234 室
yuch@sustech.edu.cn
0755-88018745
Yu Chunhong, Engineer, Department of Biology
Rm.234, No.1 Teaching building
马小英 工程师 生物系
第一教学楼 234 室
maxy@sustech.edu.cn
MA Xiaoying, Teaching Engineer, Department of Biology
Rm.234, No.1 Teaching building
生悦，工程师，生物系，
shengy@sustc.edu.cn
Sheng Yue, Teaching Engineer, Department of Biology
9.
实验员/助教、所属学系、联系
方式
Tutor/TA(s), Contact
待公布 To be announced
2
10.
选课人数限额(可不填)
Maximum Enrolment
（Optional）
无
None
11.
授课方式
Delivery Method
讲授
Lectures
习题/辅导/讨论
Tutorials
实验/实习
Lab/Practical
其它(请具体注明)
Other（Please specify）
总学时
Total
学时数
Credit Hours
16
16
32
12.
先修课程、其它学习要求
Pre-requisites or Other
Academic Requirements
无
None
13.
后续课程、其它学习规划
Courses for which this course
is a pre-requisite
无
None
14.
其它要求修读本课程的学系
Cross-listing Dept.
无
None
教学大纲及教学日历 SYLLABUS
15.
教学目标 Course Objectives
实验室安全与个人防护内容包括实验室通识安全、生物安全、化学品安全、安全设施设备、实验室规范和个人防护、实验
室废弃物处理和应急处理等，旨在帮助本科生科学理解实验室安全。
The course will introduce students to laboratory safety, including safety rules and policies, general safety, fire safety,
safety equipment, chemical safety, biological safety, waste disposal, emergency planning and incident response,
professional laboratory behaviour and personal protection. This course will improve undergraduate students’ scientific
understanding of laboratory safety.
16.
预达学习成果 Learning Outcomes
本课程完成后，学生将能够：
（1）加深对实验室安全与个人防护的理解
（2）增强安全意识
With the completion of this course, the students will
（1）gain deep understanding of laboratory safety and personal protection
（2）have a strong safety awareness
17.
课程内容及教学日历 （如授课语言以英文为主，则课程内容介绍可以用英文；如团队教学或模块教学，教学日历须注明
主讲人）
Course Contents (in Parts/Chapters/Sections/Weeks. Please notify name of instructor for course section(s), if
this is a team teaching or module course.)
3
第一次课. 实验室通识安全 4h
学习实验室安全的重要性、政策和管理规定、常见隐患，消防安全、进入实验室基本行为规范和常规防护，常见消防
安全防护品的使用。
实验部分：
1. 实验室基本行为规范培训；
2. 常见消防安全防护品的使用；
3. 消防演习。
No.1 Laboratory general safety 4h
This lecture will introduce students to the importance of laboratory safety, fire safety, general safety rules and policies,
safe lab habits, personal protective equipments and et al. Students will learn how to use some personal protective
equipments. Students will be asked to participate in fire drill/fire emergency evacuation drill.
Laboratory activities:
1. Personal hygiene and proper conduct/behavior in lab
2. How to use personal protective equipments
3. Fire drill
第二次课 化学品安全 4h
学习化学品，特别是危险化学品的分类、标签和标识、储存、废弃物处理和防护；学校危险品管理制度等；实操处理
酸碱类试剂、通风橱的使用。
实验部分：
1. 危险化学品的分类、储存、废弃物处理和防护；
2. 通风橱的使用；
3.正确处理危险试剂如酸碱。
No.2 Chemical safety 4h
This lecture will focus on chemical hazard, classification and storage of chemicals, labels, chemical waste disposal,
dangerous chemicals management in SUSTech, and et al. Students will learn how to use fume hood and avoid
unnecessary exposure to chemicals while handling dangerous chemicals, e.g. strong acids and bases.
Laboratory activities:
1. Classification and storage of chemicals
2. Chemical waste disposal
3. Fume hood operation
4. Safe handling of chemicals (i.e., acids, bases)
第三次课 生物安全 4h
了解生物危害，学习病原微生物分类、生物实验室安全等级分类、无菌技术、虚拟仿真练习 BSL-2 和 BSL-3 级实验室防
护和操作规范等。
实验部分：
4
1. 无菌技术和生物安全柜的使用；
2. 虚拟 BSL-2 级实验室防护和操作规范；
3. 虚拟 BSL-3 级实验室防护和操作规范，以及危险病毒样品意外泄露的处理模拟；
4. 虚拟 ABSL-3 级实验室漫游。
No.3 Biological safety 4h
This lecture will introduce students to biological hazard, the classification of bacterial (viral) strains and specimens
governed by the Catalogue of Pathogenic Microorganisms in China, Biosafety Levels, biological waste disposal,
professional laboratory behavior and personal protection in BSL-2 and BSL-3 labs. Students will learn how to handle
infectious substances in BSL-2, BSL-3 labs through virtual simulation experiment and aseptic technology.
Laboratory activities:
1. Aseptic technique and biological safety cabinet operation
2. Professional laboratory behavior and personal protection in BSL-2 & BSL-3 labs (virtual simulation experiments)
3. Visit ABSL-3 Laboratory (virtual simulation experiment)
第四次课 设施设备的使用 4h
了解设施设备的使用规范。学习常用安全相关设施设备（涉及高温、高压等）的操作，如常规酒精灯、电炉、烘箱、
离心机、气体钢瓶、液氮、全自动高压灭菌锅的使用等。
实验部分：学习这些设施设备的操作使用。
No. 4 Proper use of equipment 4h
This lecture will introduce students to general safety rule of equipment, especially heating machine and pressure
equipment. Students will learn to properly use alcohol burner, electric stove, forced convection oven/drying oven, gas
cylinders, liquid nitrogen, autoclaves and centrifuge.
Laboratory activities: how to operate these equipments.
第五次课 辐射安全 4h
了解辐射安全，侧重实验室常见的放射性同位素，学习放射性同位素的保存和处理等。
实验部分：虚拟仿真实验模拟同位素样品操作。
No.5 Radiation safety 4h
This lecture will focus on radiation from radionuclides most commonly used in lab. The content includes storage and
transportation of radioactive materials, disposal of radioactive waste materials, proper precautions while handling
radioactive materials and accidental spills (including contamination of working areas and pipettes with radioactive
materials).
Laboratory activities: how to handle radioactive materials (simulation experiments).
5
第六次课 实验室应急处理和急救 4h
学习常见实验室应急处理如气体中毒、触电、扎伤、烫伤、火灾等，以及急救知识。
实验部分：
1.虚拟仿真+情景模拟实验室应急处理；
2. 邀请培训机构或医生过来做急救培训（如基础急救和心肺复苏等）。
No.6 Emergency and accidents 4h
Students will learn what to do with lab accidents or emergency, including exposures to hazardous chemicals (e.g.
chemical spill, gas poisoning), contamination by pathogenic microorganisms, fire-related emergencies, scalding and
burning, electric shock and et al. Students will participate in First Aid training.
Laboratory activities:
1.
Laboratory emergencies (simulation experiments)
2. First Aid training
第七次课 参观安全体验馆 4h
No. 7 Visit Security Education Experience Platform in Shenzhen. 4h
第八次课 总结和考试 4h
No. 8 Review & Examination 4h
18.
教材及其它参考资料 Textbook and Supplementary Readings
1. 实验室生物安全，余新炳，高等教育出版社
2. Laboratory Safety Manual, University of California, Davis.
3. Guidelines for safe laboratory practices, Yale University
课程评估 ASSESSMENT
19.
评估形式
Type of
Assessment
评估时间
Time
占考试总成绩百分比
% of final
score
违纪处罚
Penalty
备注
Notes
出勤 Attendance
课堂表现
Class
Performance
10
小测验
Quiz
20
课程项目 Projects
平时作业
Assignments
6
期中考试
Mid-Term Test
期末考试
Final Exam
70
期末报告
Final
Presentation
其它（可根据需要
改写以上评估方
式）
Others (The
above may be
modified as
necessary)
20.
记分方式 GRADING SYSTEM
A. 十三级等级制 Letter Grading
√ B. 二级记分制（通过/不通过） Pass/Fail Grading
课程审批 REVIEW AND APPROVAL
21.
本课程设置已经过以下责任人/委员会审议通过
This Course has been approved by the following person or committee of authority
本课程经生物系本科教学指导委员会审议通过。
This Course has been approved by Undergraduate Teaching Steering Committee of Department of Biology.
