DNA methylation analysis from high-throughput bisulfite sequencing results


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Documentation for package ‘methylKit’ version 0.99.2

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A B C D E F G M N P R S T U misc

-- A --

adjust.methylC Adjust measured 5mC levels using 5hmC levels
adjustMethylC Adjust measured 5mC levels using 5hmC levels
adjustMethylC-method Adjust measured 5mC levels using 5hmC levels
annotate.WithFeature Annotate object with a set of genomic features
annotate.WithFeature.Flank Annotate an object with two sets of genomic features
annotate.WithGenicParts Annotate given object with gene annotation
annotateWithFeature Annotate object with a set of genomic features
annotateWithFeature-method Annotate object with a set of genomic features
annotateWithFeatureFlank Annotate an object with two sets of genomic features
annotateWithFeatureFlank-method Annotate an object with two sets of genomic features
annotateWithGenicParts Annotate given object with gene annotation
annotateWithGenicParts-method Annotate given object with gene annotation
annotationByFeature An S4 class for overlap of target features with a generic annotation
annotationByFeature-class An S4 class for overlap of target features with a generic annotation
annotationByGenicParts An S4 class for overlap of target features with gene annotation
annotationByGenicParts-class An S4 class for overlap of target features with gene annotation
assocComp Associate principal components with sample annotations

-- B --

bedgraph Get bedgraph from methylRaw, methylRawList and methylDiff objects
bedgraph-method Get bedgraph from methylRaw, methylRawList and methylDiff objects

-- C --

calculateDiffMeth Calculate differential methylation statistics
calculateDiffMeth-method Calculate differential methylation statistics
calculateDiffMethDSS calculate Differential Methylation with DSS
calculateDiffMethDSS-method calculate Differential Methylation with DSS
clusterSamples Hierarchical Clustering using methylation data The function clusters samples using 'hclust' function and various distance metrics derived from percent methylation per base or per region for each sample.
clusterSamples-method Hierarchical Clustering using methylation data The function clusters samples using 'hclust' function and various distance metrics derived from percent methylation per base or per region for each sample.

-- D --

dataSim Simulate DNA methylation data
diffMethPerChr Get and plot the number of hyper/hypo methylated regions/bases per chromosome
diffMethPerChr-method Get and plot the number of hyper/hypo methylated regions/bases per chromosome

-- E --

extract extract parts of methylRaw,methylRawDB,methylBase,methylBaseDB and methylDiff data
extract-method extract parts of methylRaw,methylRawDB,methylBase,methylBaseDB and methylDiff data

-- F --

filterByCoverage Filter methylRaw, methylRawDB, methylRawList and methylRawListDB object based on read coverage
filterByCoverage-method Filter methylRaw, methylRawDB, methylRawList and methylRawListDB object based on read coverage

-- G --

get.methylDiff get differentially methylated regions/bases based on cutoffs
getAssembly get assembly of the genome
getAssembly-method get assembly of the genome
getAssociationWithTSS Get distance to nearest TSS and gene id from annotationByGenicParts
getAssociationWithTSS-method Get distance to nearest TSS and gene id from annotationByGenicParts
getContext get the context of methylation
getContext-method get the context of methylation
getCorrelation get correlation between samples in methylBase or methylBaseDB object
getCorrelation-method get correlation between samples in methylBase or methylBaseDB object
getCoverageStats get coverage stats from methylRaw object
getCoverageStats-method get coverage stats from methylRaw object
getData get the data slot from the methylKit objects
getData-method get the data slot from the methylKit objects
getDBPath Get path to database of the methylDB objects
getDBPath-method Get path to database of the methylDB objects
getFeatsWithTargetsStats Get the percentage/count of annotation features overlapping with target features
getFeatsWithTargetsStats-method Get the percentage/count of annotation features overlapping with target features
getFlanks Get upstream and downstream adjacent regions to a genomic feature
getFlanks-method Get upstream and downstream adjacent regions to a genomic feature
getMembers Get the membership slot of annotationByFeature
getMembers-method Get the membership slot of annotationByFeature
getMethylationStats get Methylation stats from methylRaw or methylRawDB object
getMethylationStats-method get Methylation stats from methylRaw or methylRawDB object
getMethylDiff get differentially methylated regions/bases based on cutoffs
getMethylDiff-method get differentially methylated regions/bases based on cutoffs
getSampleID Get or Set Sample-IDs of the methylKit objects
getSampleID-method Get or Set Sample-IDs of the methylKit objects
getSampleID<- Get or Set Sample-IDs of the methylKit objects
getSampleID<--method Get or Set Sample-IDs of the methylKit objects
getTargetAnnotationStats Get the percentage of target features overlapping with annotation from annotationByFeature
getTargetAnnotationStats-method Get the percentage of target features overlapping with annotation from annotationByFeature
getTreatment Get or Set treatment vector of methylKit object
getTreatment-method Get or Set treatment vector of methylKit object
getTreatment<- Get or Set treatment vector of methylKit object
getTreatment<--method Get or Set treatment vector of methylKit object

-- M --

makeMethylDB coerce methylKit objects from memory to flat file database objects
makeMethylDB,methylBase-methods coerce methylKit objects from memory to flat file database objects
makeMethylDB,methylDiff-methods coerce methylKit objects from memory to flat file database objects
makeMethylDB,methylRaw-methods coerce methylKit objects from memory to flat file database objects
makeMethylDB,methylRawList-methods coerce methylKit objects from memory to flat file database objects
makeMethylDB-method coerce methylKit objects from memory to flat file database objects
methRead read file(s) to methylRaw or methylRawList objects
methRead-method read file(s) to methylRaw or methylRawList objects
methSeg Segment methylation or differential methylation profile
methSeg2bed Export segments to BED files
methylBase An S4 class for methylation events sampled in multiple experiments
methylBase-class An S4 class for methylation events sampled in multiple experiments
methylBase.obj Example methylBase object.
methylBaseDB An S4 class for storing methylation events sampled in multiple experiments as flat file database
methylBaseDB-class An S4 class for storing methylation events sampled in multiple experiments as flat file database
methylDiff An S4 class that holds differential methylation information
methylDiff-class An S4 class that holds differential methylation information
methylDiff.obj Example methylKit objects.
methylDiffDB An S4 class that holds differential methylation information as flat file database
methylDiffDB-class An S4 class that holds differential methylation information as flat file database
methylRaw An S4 class for holding raw methylation data from an alignment pipeline.
methylRaw-class An S4 class for holding raw methylation data from an alignment pipeline.
methylRawDB An S4 class for storing raw methylation data as flat file database.
methylRawDB-class An S4 class for storing raw methylation data as flat file database.
methylRawList An S4 class for holding a list of methylRaw objects.
methylRawList-class An S4 class for holding a list of methylRaw objects.
methylRawList.obj Example methylRawList object.
methylRawListDB An S4 class for holding a list of methylRawDB objects.
methylRawListDB-class An S4 class for holding a list of methylRawDB objects.

-- N --

normalizeCoverage normalize read coverage between samples
normalizeCoverage-method normalize read coverage between samples

-- P --

PCASamples Principal Components Analysis of Methylation data
PCASamples-method Principal Components Analysis of Methylation data
percMethylation get percent methylation scores from methylBase or methylBaseDB object
percMethylation-method get percent methylation scores from methylBase or methylBaseDB object
plotTargetAnnotation Plot annotation categories from annotationByGenicParts or annotationByFeature
plotTargetAnnotation-method Plot annotation categories from annotationByGenicParts or annotationByFeature
pool Pool replicates within groups to a single sample per group
pool-method Pool replicates within groups to a single sample per group
processBismarkAln Get methylation percentage from sorted Bismark alignments
processBismarkAln-method Get methylation percentage from sorted Bismark alignments

-- R --

read read file(s) to methylRaw or methylRawList objects
read.bed read a bed file and convert it to GRanges
read.bismark Get methylation percentage from sorted Bismark alignments
read.feature.flank a function to read-in genomic features and their upstream and downstream adjecent regions such as CpG islands and their shores
read.transcript.features Read transcript features from a BED file
readBed read a bed file and convert it to GRanges
readBed-method read a bed file and convert it to GRanges
readFeatureFlank a function to read-in genomic features and their upstream and downstream adjecent regions such as CpG islands and their shores
readFeatureFlank-method a function to read-in genomic features and their upstream and downstream adjecent regions such as CpG islands and their shores
readTranscriptFeatures Read transcript features from a BED file
readTranscriptFeatures-method Read transcript features from a BED file
reconstruct Reconstruct methylBase or methylBaseDB object based on a new methylation percentage matrix
reconstruct-method Reconstruct methylBase or methylBaseDB object based on a new methylation percentage matrix
regionCounts Get regional counts for given GRanges or GRangesList object
regionCounts-method Get regional counts for given GRanges or GRangesList object
removeComp Remove principal components from a methylBase object
removeComp-method Remove principal components from a methylBase object
reorganize Reorganize methylKit objects by creating new objects from subset of samples
reorganize-method Reorganize methylKit objects by creating new objects from subset of samples

-- S --

select selects rows from of methylKit objects
select-method selects rows from of methylKit objects
selectByOverlap selects records of methylDB objects lying inside a GRanges range
selectByOverlap-method selects records of methylDB objects lying inside a GRanges range
show,methylBase show method for methylKit classes
show,methylBaseDB show method for methylKit classes
show,methylDiff show method for methylKit classes
show,methylDiffDB show method for methylKit classes
show,methylRaw show method for methylKit classes
show,methylRawDB show method for methylKit classes
show,methylRawList show method for methylKit classes
show,methylRawListDB show method for methylKit classes
show-method show method for methylKit classes

-- T --

tileMethylCounts Get methylated/unmethylated base counts for tilling windows
tileMethylCounts-method Get methylated/unmethylated base counts for tilling windows

-- U --

unite unite methylRawList to a single table
unite-method unite methylRawList to a single table
updateMethObject update methylKit objects The method updates object from earlier versions (<v0.9.1) to latest object.

-- misc --

[-method extract parts of methylRaw,methylRawDB,methylBase,methylBaseDB and methylDiff data